A novel fluorescent sensor, 7-hydroxy-4-methylcoumarin-8-carbaldehyde-(fluorescein) hydrazone was designed and synthesized for selective recognition of Zn 2+ in HEPES buffer medium of PH 7.4. This reagent could be used as a probe for Zn 2+ by monitoring changes in the absorption and the fluorescence spectral patterns. More importantly, this sensor displays an extreme selectivity, sensitivity and color change for Zn 2+ over other earth- and transition metal ions, which was mainly due to the spirolactam ring-opening power of Zn 2+ . Upon the addition of Zn 2+ , an overall emission change of 33-fold was observed and the detection limit was low as 6.54 ppb. Photoinduced electron transfer process, coupled with the intramolecular charge transfer process, are proposed to explain the observed spectral response.