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Displaceable binding of [ 3 H]forskolin to human platelet membranes can be detected in the presence of magnesium. There is an increase in the number of [ 3 H]forskolin binding sites when membranes are incubated with GppNHp or NaF in the presence of magnesium. Prostaglandin E 1 , which stimulates human platelet adenylate cyclase, does not affect the binding of [ 3 H]forskolin...
The effects of synthetic rat adrenomedullin (rAM), a novel vasorelaxant peptide originally isolated from human pheochromocytoma, on receptor binding and cAMP generation were studied in cultured rat vascular smooth muscle cells (VSMC). A binding study using [ 125 I]rAM revealed the presence of a single class of high-affinity (K d 1.3 x 10 -8 M) binding sites for...
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