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Exocytosis induced by crosslinking the type I receptor for Fc e , domains present on rat mucosal mast cells (RBL-2H3-line) requires the influx of Ca 2+ ions and is markedly influenced by the concentration of monovalent cations (K + , Na + and protons) in their medium. We investigated the role of these ions in coupling the immunological stimulus to secretion using...
Rat basophilic leukemia (RBL-2H3) cells, which exhibit Ca 2+ -dependent secretion of granules when stimulated with antigen or the Ca 2+ -ionophore A23187, were observed under a video-enhanced light/fluorescence microscope. Exocytotic events of individual granules were visualized in individual cells stimulated with antigen or A23187. Exocytosis of granules stimulated with...
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