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Pertussis toxin was found to stimulate catecholamine release from bovine adrenal chromaffin cells in a Ca 2+ -dependent manner and in the absence of any stimulatory or inhibitory agonists for this cell. The release of catecholamine was associated with the ADP-ribosylation of an approx. 40 kDa protein present in the total membrane fraction. These results are consistent with the existence...
The hypothesis that inhibition of secretion by botulinum neurotoxin type D occurs by an intracellular process involving ADP-ribosylation has been directly tested by measuring both the extent of inhibition of secretion and of ADP-ribosylation in the same cells. Although the inhibitory effect of unpurified toxin closely parallels intracellular ribosylation, the two events are clearly unrelated, as using...
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