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We show that MDCK I cells express, besides the classical (Na + +K + )ATPase, a Na + -stimulated ATPase activity with the following characteristics: (1) K 0.5 for Na + 7.5±1.5mM and V max 23.12±1.1nmol Pi/mg per min; (2) insensitive to 1mM ouabain and 30mM KCl; and (3) inhibited by furosemide and vanadate (IC 50 42.1±8.0 and 4.3±0.3μM, respectively)...
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