The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
The vast majority of proteins in the nematode cuticle are collagens. Cuticular collagen-encoding genes (col) have been described for the animal parasites Ascaris suum and Haemonchus contortus and for the free-living Caenorhabditis elegans. The proteins encoded by all these genes seem to have the same basic structure, indicating that there is a conserved subfamily of cuticular col in these nematodes...
In order to obtain the 5 ends of the three mouse calmodulin (CaM) cDNAs, we modified the standard 5 RACE (rapid amplification of cDNA ends) method to use degenerate synthetic oligodeoxyribonucleotides to prime cDNA synthesis of all three CaM mRNAs. In this modified method, the degenerate primers were annealed to mRNAs in an incubation step prior to the reverse transcription reaction. Separating...
Here we describe the cloning of a full-length cDNA encoding a neutrophil chemoattractant, peptide ENA-78, from human platelets. The cDNA encodes a predicted sequence of 114 amino acids and contains the Cys motif C-X-C found in other members of the α-chemokine family which also includes interleukin 8 (IL-8). ENA-78 has a high degree of sequence identity with other platelet-derived chemokines which...
The mutation spectrum attributable to an antimutator DNA polymerase in a eukaryotic cell was examined. Drug-resistant thymidine kinase (tk) mutants derived from both a wild-type (wt) strain, KOS and an antimutator DNA polymerase (pol) mutant, PAA r 5, of herpes simplex virus (HSV), were isolated, and the mutated tk genes were characterized at the sequence level. While both transition and frameshift...
The cDNAs encoding the porcine 65- and 67-kDa glutamic acid decar☐ylases (GAD 65 and GAD 67 , respectively) were cloned by the PCR method. The 2246-nucleotide (nt) GAD 65 cDNA contained an open reading frame (ORF) coding for a protein of 585 amino acids (aa), and the 3262-nt GAD 67 cDNA contained an ORF coding for a protein of 594 aa. A remarkable conservation was shown...
A 6.3-kb DNA fragment encoding two eukaryotic-type serine/threonine protein kinases (Ser/Thr PK) was cloned from Streptomyces coelicolor A3(2) by using a PCR product obtained with primers based on highly conserved regions of eukaryotic Ser/Thr PK. The nucleotide (nt) sequence of the essential 4.4-kb fragment contained two possible ORFs. One ORF (PkaA) contained 543 amino acids (aa), while another...
A genomic DNA encoding a mouse adrenocorticotropin (ACTH) receptor was isolated. The predicted 296-amino-acid sequence showed 88.9 and 78.7% identities to the human and bovine homologues, respectively.
An examination of mRNA expression of the serotonin genes encoding both ψ5HT 1Dα and the related 5HT 1Dα , demonstrated that the pseudogene is transcribed, and has a tissue distribution similar to the 5HT 1Dα receptor-encoding gene. This ψ5HT 1Dα transcript is capable of being translated into a polypeptide of only 28 amino acids...
The human dopamine D5 receptor (DRD5) gene family consists of the DRD5-encoding gene (DRD5) and the pseudogenes ψDRD5-1 and ψDRD5-2. Analysis of the 5 UTR of DRD5 and homologous regions in the pseudogenes revealed that the nucleotide identity (approx. 95%) extended for 1.9 kb and terminated at a monomeric Alu sequence in each of the pseudogenes. The presence of Alu sequences in the pseudogenes,...
The human isoleucyl-tRNA synthetase (IRS)-encoding cDNA, whose primary structure we report here, has an open reading frame (ORF) which encodes a protein of 1262 amino acids (aa) with strong homology to IRS from yeast (53.5%) and Tetrahymena (51.0%) and contains all the major consensus motifs of class-I hydrophobic amino-acyl-tRNA synthetases (aaRS; MRS, LRS, VRS, IRS). However, the human enzyme has...
The nucleotide (nt) sequence of the human cDNA encoding PAP, a pancreatic secretory protein induced during acute pancreatitis, was found to be identical with that of a gene activated in human primary hepatocellular cancer, designatedHIP . To obtain insight into the expression of PAP/HIP, we characterized the gene organization, especially focusing on the 5 -flanking region, and found that it spans...
Partial cDNA clones generated by RT-PCR were used as probes to clone the cDNAs encoding the human α4 and β2 neuronal nicotinic acetylcholine receptor (nAChR) subunits. The 2.1-kb α4 cDNA shows 84 and 76% identity to the rat and chicken cDNA sequences, respectively. The deduced amino-acid sequence shares 89 and 84% similarity, respectively, with the corresponding rat and chicken proteins, with most...
Troponin T (TpnT), an essential component of the Ca 2+ -regulatory troponin complex, is involved in protein-protein interactions with other thin-filament proteins during muscle contraction in vertebrate striated muscle (VSM). The isoforms of TpnT are encoded by members of a multigene family which, by alternate splicing, produces a complex pattern of isoproteins in VSM. The functional...
Using the polymerase chain reaction with degenerate oligo primers we identified six new Antennapedia-like homeobox sequences expressed in a regenerating planarian. cDNA fragments containing the entire homeobox sequences for four of these genes were obtained. Two of them, Dutarh-3 and Dutarh-4, belong to the classical Antennapedia type and display extensive identity with Antennapedia- and Deformed-type...
Micrometastases of solid tumors are most commonly detected by immunocytochemistry using monoclonal antibodies directed against tissue-specific gene products like cytokeratin-18 (CK-18) and the carcinoembryonic antigen (CEA). While CK-18 is a marker for epithelia in general, CEA is mainly employed in the detection of gastrointestinal and breast carcinomas. To improve the sensitivity and specificity...
The apolipoprotein B-encoding gene (apoB) 3 end hypervariable repeats contain 11- to 16-bp A + T-rich sequences and are organized tandemly. Sixteen alleles varying in repeat number (26, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55 and 58) were sequenced. Sequencing revealed a gradual extension of the number of bd (5 -TTTTATAATTAAAATA TTTATAATTAAATA) repeats [the fourth and fifth repeat...
In order to examine ribozyme (Rz) activity in vivo, we have adapted a virus to deliver Rz to plants. DNA fragments that code for both active and mutant cis-hairpin Rz were cloned into the double-stranded DNA plant virus, cauliflower mosaic virus (CaMV). These Rz constructs successfully infected Brassica campestris rapa (turnip). The plants that were infected with the active-Rz construct showed,...
A previously unreported 196-bp PstI fragment was found in intron 1 of the gene encoding chicken growth hormone (cGH) when a PCR assay for an MspI restriction fragment length polymorphism was established. A pair of PCR primers was designed according to the published cGH sequence and used to amplify a fragment which contained two MspI sites, one polymorphic and another non-polymorphic. However, amplification...
NAD-dependent formate dehydrogenase (FDH) was isolated from Candida methylica (Cm) grown on 0.5% methanol. Its N-terminal amino acid (aa) sequence was determined, as was that of a commercial FDH from Candida boidinii. Degenerate oligodeoxyribonucleotides were made to the 5' region of the fdh gene from Cm using this information and to the 3' region using C-terminal aa sequence data from the methylotropic...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.