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Arachidonate 8-lipoxygenase was identified in phorbol ester induced mouse skin. We expressed the enzyme in an Escherichia coli system using pET-15b carrying an N-terminal histidine-tag sequence. The enzyme, purified by nickel–nitrilotriacetate affinity chromatography, showed specific activity of about 0.1μmol/min/mg of protein with arachidonic acid as a substrate. When metabolites of arachidonic acid...
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