Escherichia coli strain XL1 blue (pTrcfuc) was used to overexpress fuculose-1-phosphate aldolase (Fuc-1-phosphate) in a fed-batch bioreactor operating at constant specific growth rate, 0.1h - 1 . Maximum dry cell weight concentration in the fermentor was 45gdm - 3 , over this biomass concentration oxygen became limiting. Once the recombinant strain behaviour was characterised under fixed growth conditions, optimal induction conditions to maximise the recombinant protein productivity in the system was studied. Time after induction to achieve maximum specific activity was around 11h. Best results obtained were: specific activity of 1100AUg - 1 DCW, enzyme concentration of 47,000AUdm - 3 (around 3gdm - 3 ) and global volumetric productivity of 1200AUdm - 3 h - 1 .