To study the chemical components of the seeds of Caesalpinia sappan. Compounds were isolated and purified by column chromatography. Their structures were elucidated on the basis of spectroscopic methods. In addition, the cytotoxic activity of these compounds were evaluated using SF-268, MCF-7 and HepG2 cell lines by the MTT assay. Four cassane diterpenoids were obtained and their structures were identified as phanginin L (1), phanginin M (2), phanginin I (3), phanginin G (4). Phanginin L (1) and phanginin M (2) are new cassane diterpenoids. Compound 1 showed moderate cytotoxicity against HepG2 cell line with IC 50 of 9.13 μg·mL −1 .
Financed by the National Centre for Research and Development under grant No. SP/I/1/77065/10 by the strategic scientific research and experimental development program:
SYNAT - “Interdisciplinary System for Interactive Scientific and Scientific-Technical Information”.