An immobilization technique (using nanotechnology) that imparts psychrostability and enhanced activity to a psychrophilic pectate lyase (PL) has been described here. Pectate lyase from a pyscrophile was supplemented with calcium hydroxyapatite nanoparticles (NP-PL) as a substitute for Ca, (the cationic activator of this enzyme) and entrapped in single walled nanotube (SWNT) based molecular self-assembly. The activity and stability of PL was enhanced both at temperatures as low as 4°C and as high as 80°C in presence of NP and SWNT. The enzyme could be repeatedly released and re-trapped (in SWNT based molecular self-assembly) while retaining significant activity. The immobilized PL (in SWNT based molecular self-assembly), retained its activity after repeated freezing and thawing. This unique capability of SWNT to activate and stabilize a cold active enzyme at temperatures much lower or higher than its optimal range may be utilized for processes that require bio-conversion at low temperatures while simultaneously allowing for shifts to higher temperature.
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