Polish Journal of Microbiology
The possibility of using mineral oils as a carbon source by bacteria adapted to high oil concentrations was tested in liquid media with different pH values (pH = 5,7 and 9). Two types of inocula were tested: inoculum I consisted of selected strains used in the bioremediation of oil-contaminated soils and inoculum II contained bacteria isolated from soil samples previously bioremediated at pH = 5,...
The paper presents the activity of anaerobic bacterial communities isolated from soil polluted by aircraft fuel on distillery decoctions with phosphogypsum. The microorganisms were selected using the microcosms method, and then enriched on Postgate medium with ethanol. The isolated communities became the inoculum to establish a culture on potato and rye distillery decoctions. The obtained results...
Candida sp. may be regarded as one of the leading etiologic agents of hospital-acquired infections, including those related with the indwelling medical devices, which become colonized by the yeasts, accompanied by biofilm formation. In this paper we assayed in vitro susceptibility to caspofungin of planktonic and sessile cells of nasopharyngeal isolates of Candida sp. Two types of biomaterials were...
Considering the role of lectin-carbohydrate interactions between Helicobacter pylori bacteria and the host cells we addressed the question on how mannose binding lectin - MBL, present in human plasma, may influence the phagocytosis of H. pylori by peripheral blood granulocytes. For phagocytosis assay the granulocytes separated from peripheral blood of healthy H. pylori-seronegative donors were used...
Urinary tract infections are frequently caused by Proteus mirabilis strains. In the previous studies there were defined the complete structures of O-polysaccharide parts of lipopolysaccharides from strains: P. mirabilis O3 (S1959), P. mirabilis O9 and P. mirabilis O18. In the present study it was investigated bactericidal effect of normal human serum (NHS) to P. mirabilis strains. We also focused...
In this study the biodegradation of oil by hydrocarbon degrading Pseudomonas putida in the presence of a biosurfactantproducing bacterium was investigated. The co-culture of test organisms exhibited improved degradation capacities, in a reproducible fashion, in aqueous and soil matrix in comparison to the individual bacterium culture. Results indicate that the in situ biosurfactant production not...
The aim of this research was to develop a suitable method of succinate dehydrogenase activity assay in situ for different industrial yeast strains. For this purpose different compounds: EDTA, Triton X-100, sodium deoxycholate, digitonin, nystatin and β-mercaptoethanol were used. The permeabilization process was controlled microscopically by primuline staining. Enzyme assay was conducted in whole yeast...
We investigated the regulation of the cjaA and cjaB genes of Campylobacter coll. These genes are seemingly arranged into one operon but appear to encode functionally different proteins i.e. an extracytoplasmic solute receptor and a MHS - metabolite: H⁺ symporter transport protein. Analysis of various transcriptional cjaA and/or cjaB lacZ fusion constructs revealed that both genes are arranged in an...
A total of 90 Pseudomonas aeruginosa strains isolated from 4 hospitals in the west-north region of Poland were studied by arbitrarily primed polymerase chain reaction (AP-PCR). AP-PCR results revealed the presence of 11 main groups of patterns (A-K) and 5 unique patterns among isolates. Generally, they were characterized by high resistance to antibiotics tested and significant differences in serogroups...
Nine strains belonging to Bacillus cereus group has been isolated from food and environmental samples. Their taxonomic position was confirmed by RFLP analysis of 16S rRNA gene digested with Taq\. The detection of DNA sequences encoding the hemolysin BL complex and enterotoxin NHE, was studied in Bacillus sp. isolates. Set of primers was used to amplify fragment of hblD gene by PCR. For the detection...
An association between Helicobacter infection and autoimmune hepatitis (AIH) in children was investigated. The prevalence of antibodies to H. pylori did not differ between the AIH and the control group, (22% versus 14%), and antibodies to non-gastric Helicobacter were not detected in either group. H. pylori DNA was found in two AIH liver tissues, but Helicobacter was not cultured from any sample.
A marine Bacillus strain was isolated from the eastern harbour of Alexandria and identified as Bacillus sp. MIG. Maximum activity of studied proteases was obtained when the bacterium was grown in medium with 1 % wheat bran and 0.5% yeast extract in addition to the mineral salts and incubated for 48 h at 30°C and 120 rpm. Two alkaline proteases (Pro 1 and Pro 2) were purified to homogeneity using cation...
Because dilution plating is more labor intensive than direct plating, we would like to propose the use of a direct plating technique for estimation of Penicillium verrucosum abundance in cereal grain in incubation studies, with use of very selective and indicative for the fungus agar DYSG medium. The proposed method is based on the measurement of the diameter of P. verrucosum colonies grown around...
Studies were carried out on the microflora of phyllosphere and soil rhizosphere of hulled (Chwat variety) and naked (Akt variety) oats. The material taken for study embraced samples of leaves and soil rhizosphere taken from cultivations differing in extent of nitrogen fertilization. The studies involved determination of total number of aerobic heterotrophic bacteria belonging to the genus Pseudomonas...
Only 9 (11.2%) out of 80 studied bacterial strains were able to utilize iron saturated 2-oxo acids and hydroxyacids and grow on o-phenantroline containing media. These strains belonged to Enterococcus faecalis and Enterococcus faecium species and were isolated from clinical material. Iron sources utilized by all of these strains were Fe(III) complexes with pyruvic, 2-oxobutyric, 4-methylthio-2-oxobutyric,...
The iron-oxidizing bacteria Acidithiobacillus ferrooxidans is an example of strictly chemolitotrophic extremophile occurring in acidic environments. The prime niche of these microorganisms is an environment with low pH and high concentrations of iron, sulfide minerals or sulfur. Besides these environments, A. ferrooxidans is also isolated from heavy metal contaminated environments such as soil and...
The use of microbial exopolysaccharides (EPS) in the food, pharmaceutical, and chemical industries has steadily increased during the past decade. A bioactive EPS producing microorganism, Streptomyces nasri was isolated from Kuwait tropical soil and the proteopolysaccharide was tested for its antimicrobial activity. The isolate was subjected to ultraviolet (UV) radiation and acridine orange (AO) treatment...
L-forms of S. epidermidis were induced at 35°C with the use of an L-form medium with penicillin. The aim of this study was to evaluate the frequency of L-form induction and demonstrate whether the origin of the clinical strains affects the frequency of L-forms induction, as well as to study whether the time of action of the antibiotic has an influence on frequency of L-form induction.