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The biotransformation of phosphogypsum in cultures of sulfate-reducing bacteria (SRB) isolated from crude petroleum-refining wastewaters or purified using activated sludge method was studied. Selection was with the microcosms method on Postgate and minimal medium with different carbon sources, Emerson medium and petroleum-refining wastewaters. Highest hydrogen sulfide production, in excess of 500...
The paper presents the activity of anaerobic bacterial communities isolated from soil polluted by aircraft fuel on distillery decoctions with phosphogypsum. The microorganisms were selected using the microcosms method, and then enriched on Postgate medium with ethanol. The isolated communities became the inoculum to establish a culture on potato and rye distillery decoctions. The obtained results...
The activity of photosynthesizing sulfur bacteria in a continuous culture was studied. The bacteria were isolated from the natural environment with the use of the Winogradski column. Isolated bacteria were cultured in synthetic medium and in the effluent from the column containig HS-. Sulphides, the main product of reduction of sulfates in phosphogypsum, were used by green sulfur bacteria in the photosynthetic...
Three independent 28 or 32-day stationary cultures of Desulfotomaculum acetoxidans DSM 771 strain were carried out under anoxic conditions in acetate or lactate-containing media. The acids were the sole carbon and energy sources in these media. During cultivation the turbidity (for calculation of cell division index) and hydrogen sulfide contents were determined in culture broth and reduced glutathione...
Earlier research demonstrated the secretion of benzoate, which must be oxygenated to its 4-hydroxy derivative in order to be included in further sulfate uptake processes. The present study on Desulfotomaculum acetoxidans DSM 771 was designed to determine the activity and catalytic specificity of the enzyme (most probably peroxidase) catalyzing the hydroxylation of secreted benzoate. Peroxidase activity...
The utilization of pig manure as a source of nutrients for the dissimilatory reduction of sulfates present in phosphogypsum was investigated. In both types of media used (synthetic medium and raw pig manure) increased utilization of sulfates with growing COD/SO₄²⁻ ratio in the medium was observed. The percent of sulfate reduction obtained in synthetic medium was from 18 to 99%, whereas the value for...
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