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The aim of the study was to determine the degree of phagocytic activity of neutrophils, lymphocyte histogenesis and total immunoglobulin level in six bitches with glandular cystic hyperplasia - pyometra complex (GCH - PC) before and after ovariohysterectomy in comparison to healthy dogs. Haematological study showed significant increases (P<0.005) in the white blood cell count and neutrophilia....
The first Aujeszky's disease (AD) vaccination - eradication programme in Poland was introduced in two different in size farrow-to-finish farms with a high percentage of latently infected pigs. All the pigs were vaccinated with gE- and TK-negative vaccine Nobi-Porvac Aujeszky Live Begonia (Intervet, Holland) according to the following schedule: breeding stock twice with a 4-week interval and then 3...
Melatonin secretion is not regulated via simple negative feedback inhibition. However some results show that melatonin may influence its own synthesis and secretion as well as other processes in the pineal gland. The present study was undertaken to check the effects of melatonin administered at different times of day on the ultrastructure of pig pinealocytes. The study was performed in summer under...
The study was carried out on three groups of cows kept in the systems: I - indoor and pasture, II - indoor with a run, III - indoor. The experiment lasted one year and involved summer and winter feeding. Contents of vitamin A and E in blood serum were determined. The highest serum levels of vitamin E were determined in animals kept in both indoor + pasture and indoor + run systems. The system of keeping...
The aim of the studies was the use of Haemophilus somnus vaccine in the immunization of chickens to protect them against Salmonella infections by increasing the local cross-immunity of the alimentary tract. Broilers were immunized with a live vaccine administered in drinking water on the 1st and the 14,h day of life. The groups were as follows: H. somnus (24h broth culture, 109 cfu/ml); Salmonella...
This study investigated an influence of oestradiol and progesterone on expression of LH/hCG receptors in the porcine uterine broad ligament. The tissues were obtained from nineteen ovariectomized crossbred gilts. The animals received i.m. injections of 2 ml of corn oil (control; n=4), 25 μg kg-1 of oestradiol benzoate (n=5), 500 μg kg-1 of progesterone (n=5) or 25 μg kg-1 of oestradiol benzoate with...
Single-, double- and triple-labelling immunohistochemistry were applied to study the distribution and neurochemical properties of cholinergic nerve fibres supplying the vas deferens in juvenile and adult pigs. Cholinergic nerves were identified using an antibody against choline acetyltransferase. Immunoblotting was applied to verify the specificity of choline acetyltransferase immunostaining. Western...
This study investigated the influence of active immunisation against Gn-RH on adrenergic innervation of the porcine testis, epididymis and vas deferens. Seven groups of the boars were used including three control groups (n=9) Gl, G2 and G3 consisting of non-immunised animals aged 10,18 and 26 weeks, respectively, and four groups of immunised pigs (7-9 animals in each group). All the experimental boars...
The aim of this study was to determine histotopography and age-related changes of immunoreactive substance P (SP-IR) nerve plexus in dog cornea. In this research corneas of three groups of dogs of different age were used: young (2-3 months), adult (1-3 years) and old (8 and more years). Immunohistochemical demonstration of SP was performed on 40 um serial free-floating corneal sections cut parallel...
The aim of this study was an assessment of the immune response of cats experimentally infected with M. canis and a determination of the extent of immunity acquired after recovery. Studies were carried out on 24 domestic, short-haired cats, aged 3-4 months. The resistance of the animals was assessed by reinfection, and the degree of immune response. It has been found that after recovery from an experimental...
The aim of this study was to determine the influence of unilateral, intraovarian infusions of Escherichia coli endotoxin (LPS, lipopolysaccharide, serotyp 055:B5) on the concentration of progesterone (P4), testosterone (T), estradiol-17ß (E2) and luteinizing hormone (LH) in peripheral and ovarian blood plasma of gilts. The morphology of ovaries was also estimated. Fourteen sexually matured gilts with...
The in vitro effects of iridovirus isolated from catfish on head kidney lymphocyte activity were examined in carp, rainbow trout and sheatfish. Mitogenic proliferative assay was used to demonstrate an immunosuppressive effect of iridovirus on the proliferative response of head kidney lymphocytes stimulated by ConA and LPS. The production of IL-2-like protein by head kidney lymphocytes stimulated by...
Four groups of turkeys were fed mixtures containing fat of different peroxide values, 5, 50, 100 and 150 mEq O2/kg, respectively, for four months; the fat content over successive months was 2, 3, 4 and 5%. Every month blood samples were collected from 12 birds randomly selected from each group. Moreover, six turkeys from each group were slaughtered to collect samples of the liver, breast muscles and...
This stiřdy was aimed at determining the influence of three different freezing methods on the horsemeat microfloraduring a 3-month-cold storage. The three methods used included freezing in a container ventilation chamber, freezing in liquid carbon dioxide and two-stage freezing method (combined method based on using liquid carbon dioxide and freezing in a container ventilation chamber). The study...
This study attempts to determine the subpopulations of peripheral blood lymphocytes in horses with a panel of already described clusters of differentiation on equine leukocytes. Blood specimens from 21 healthy horses were examined by lymphocyte immunophenotyping with fluorochrome labelled monoclonal antibodies and flow cytometry. In the population of horses investigated, the percentage of EqCD2+ T...
The objective of this study was to examine the effects of 100, 300 and 500 MPa hydrostatic pressures on the morphology of muscle fibres and texture (tenderness and toughness) of the muscles Longissimus thoracis and Gluteus medius taken from chilled carcasses of Black-and-White cattle and Polish Large White pig. Histological examination, scanning electron microscopy (SEM), morphometric analysis (indices...
Lymphocytes of haematologically and serologically EBB-positive cows were isolated and incubated with CD4, CD8, Cdllc and alpha bovine IgM markers, produced by Int. ILRAD Peter Maesseus. The cells were analysed immediately following fluorescein marking, in flow cytometer (FACS Calibur - Becton Dickinson). The number of proliferating B lymphocytes with increased IgM marker expression was high, testifying...
The aim of this study was to determine whether pathological changes in Escherichia coli endotoxin (LPS, lipopolysaccharide)-infused ovary are associated with an increase in NO production, and whether inflammatory mediators produced by LPS-infused ovary can affect the contralateral one to stimulate NO synthesis. Therefore, the activity of NADPH-diaphorase (NADPH-d) and distribution of the inducible...
This study investigated the influence of active immunization against GnRH on the innervation of the prostate gland in pigs by means of immunohistochemical and quantitative methods. Three control groups of the animals including: Gl- 10 week-old, G2- 18 week-old, G3- 26 week-old and four groups of immunized pigs: G4, G5, G6 and G7 (from 7 to 9 animals in each group) were used. Immunization was accomplished...
The activity of monoamine oxidase (MAO) in cow serum undergoes considerable fluctuations in the course of the oestrous cycle. The aim of the study was to determine the activity of MAO and its isoenzymes A (MAO-A) and B (MAO-B) in cow serum near the parturition. The experiments were performed on 8 pregnant and clinically healthy cows of the Polish Black and White Breed. Peripheral blood samples were...
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