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The investigation was carried out on 51 piglets. 11 piglets were used as control animals. The remaining 40 piglets were given (daily, starting from the third day of life) 2 ml of 18-hour Lactobacillus strain culture containing 1 x 108 bacteria isolated from the pig( alimentary tract. Clinical, haematological and biochemical examinations were performed on all animals at the age of 2, 6, 15 and 21 days...
The following paper deals with the results of a long-lasting cannulation of the porcine pancreas performed using new methods. One method is based on a direct cannulation of the pancreatic duct after its incision. The other (designed by the authors) consists of a cannulation of the pancreas through the minor duodenal papilla. During the 14-day experimental period the efficiency of both methods was...
The pattern of distribution of nerve fibers containing vasoactive intestinal polypeptide (VIP) and neuropeptide Y (NPY) in the oviduct and uterine horn was compared in ovariectomized (OVX) gilts administered oestradiol or placebo. Compared to controls, VIP-positive nerve fibers were scarce in the muscular layer and submucosa and absent under the luminal epithelium of the oviduct and uterine horn by...
The efficacy of zinc application in the treatment and prevention of diarrhoea in the weaning period of piglets has been demonstrated. However, beside this positive effect some authors suggested the possibility of harmful effects of zinc oxide supplementation on the environment and public health. The aims of the study were to evaluate, in experimental and field conditions, the influence of different...
In the present paper the influence of the rat C-terminal fragment [Tyr°]CGRP(28-37) (Ct-CGRP) was compared with the influences of noradrenaline (NA) and acetylcholine (ACh), respectively on isolated pig uterine artery (UA). It was shown that Ct-CGRP (10 -8 mol/1) caused contraction of UA, similar to NA (10-7 mol/1) action. ACh addition before Ct-CGRP treatment inhibited the action of this peptide...
Pharmacological analysis of C-terminal fragment of Calcitonin Gene-related Peptide [Tyr0]CGRP(28-37) (Ct-CGRP) in isolated pig uterine artery (UA) collected on days 17-18 of the oestrous cycle were studied. It was found that inhibition of a-adrenergic receptor activity (with phentolamine, 10-3 mol/1) inhibited the vasocontractile reaction of Ct-CGRP (10-8 mol/1), while ß-adrenergic receptors blockade...
Two ELISA kits for diagnosis of Brucella suis infections in pigs and hares were evaluated. The evaluation was performed by comparison of the results obtained in the ELISA with those in traditional serological methods. To evaluate the ELISA for testing pig sera, 100 samples from pigs from infected herds, 40 samples from pigs from doubtful herds and 18 672 sera from healthy pigs monitored for brucellosis...
Molecular probes were generated to study the expression of tyrosine hydroxylase (TH), choline acetyltrans- ferase (ChAT), galanin (GAL), substance P (SP) and calcitonin gene-related peptide (CGRP) in the peripheral nervous system of the pig with in situ hybridization. Application of the probes to the sections from the inferior mesenteric ganglion (IMG) in control animals revealed numerous neurons...
Local counter current transfer of substances between venous and arterial vessels has been found in the perihypophyseal vascular complex after administration into the supraorbital vein. The present experiments investigate whether similar transfer could be found after nasal administration of testosterone. Experiments were conducted on the model of isolated pig's head perfused with autologous blood through...
The broad ligament and .'paraovarian sac tissuek in various areas of the paraovarian lymphatic plexus after administration of carbon particles and latex bieads were examined for absorption from peritoneal and sac cavities in pigs during the estrous cycle. 30 min after the introduction of carbon particles, lymphatic pathways were stained black through all phases of the estrous cycle, however, near...
The first Aujeszky's disease (AD) vaccination - eradication programme in Poland was introduced in two different in size farrow-to-finish farms with a high percentage of latently infected pigs. All the pigs were vaccinated with gE- and TK-negative vaccine Nobi-Porvac Aujeszky Live Begonia (Intervet, Holland) according to the following schedule: breeding stock twice with a 4-week interval and then 3...
Melatonin secretion is not regulated via simple negative feedback inhibition. However some results show that melatonin may influence its own synthesis and secretion as well as other processes in the pineal gland. The present study was undertaken to check the effects of melatonin administered at different times of day on the ultrastructure of pig pinealocytes. The study was performed in summer under...
This study investigated an influence of oestradiol and progesterone on expression of LH/hCG receptors in the porcine uterine broad ligament. The tissues were obtained from nineteen ovariectomized crossbred gilts. The animals received i.m. injections of 2 ml of corn oil (control; n=4), 25 μg kg-1 of oestradiol benzoate (n=5), 500 μg kg-1 of progesterone (n=5) or 25 μg kg-1 of oestradiol benzoate with...
Single-, double- and triple-labelling immunohistochemistry were applied to study the distribution and neurochemical properties of cholinergic nerve fibres supplying the vas deferens in juvenile and adult pigs. Cholinergic nerves were identified using an antibody against choline acetyltransferase. Immunoblotting was applied to verify the specificity of choline acetyltransferase immunostaining. Western...
This study investigated the influence of active immunisation against Gn-RH on adrenergic innervation of the porcine testis, epididymis and vas deferens. Seven groups of the boars were used including three control groups (n=9) Gl, G2 and G3 consisting of non-immunised animals aged 10,18 and 26 weeks, respectively, and four groups of immunised pigs (7-9 animals in each group). All the experimental boars...
The aim of this study was to determine the influence of unilateral, intraovarian infusions of Escherichia coli endotoxin (LPS, lipopolysaccharide, serotyp 055:B5) on the concentration of progesterone (P4), testosterone (T), estradiol-17ß (E2) and luteinizing hormone (LH) in peripheral and ovarian blood plasma of gilts. The morphology of ovaries was also estimated. Fourteen sexually matured gilts with...
The objective of this study was to examine the effects of 100, 300 and 500 MPa hydrostatic pressures on the morphology of muscle fibres and texture (tenderness and toughness) of the muscles Longissimus thoracis and Gluteus medius taken from chilled carcasses of Black-and-White cattle and Polish Large White pig. Histological examination, scanning electron microscopy (SEM), morphometric analysis (indices...
The aim of this study was to determine whether pathological changes in Escherichia coli endotoxin (LPS, lipopolysaccharide)-infused ovary are associated with an increase in NO production, and whether inflammatory mediators produced by LPS-infused ovary can affect the contralateral one to stimulate NO synthesis. Therefore, the activity of NADPH-diaphorase (NADPH-d) and distribution of the inducible...
This study investigated the influence of active immunization against GnRH on the innervation of the prostate gland in pigs by means of immunohistochemical and quantitative methods. Three control groups of the animals including: Gl- 10 week-old, G2- 18 week-old, G3- 26 week-old and four groups of immunized pigs: G4, G5, G6 and G7 (from 7 to 9 animals in each group) were used. Immunization was accomplished...
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