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Critical transport velocity, thickness, width, length, angle of sliding friction and weight of small−leaved lime (Tilia cordata Mill.) seeds from five batches, harvested in four neighboring seed regions in Poland, were determined. The values of shape factor, cross−sectional area, volume and density were calculated for each seed. The results were compared using standard statistical methods (simple...
Cryopreservation is a method of storage of biological material at the temperature of liquid nitrogen (–196°C, LN). The main advantage of this method is the possibility to store viable cells for a long time. Desiccation and freezing sensitivity of seeds, their fragments or other plant organs, which are useful as genetic resources, should be investigated before cryopreservation.
Cryopreservation in liquid nitrogen (–196°C, LN) is the method of long−term conservation of plant tissues, which has been evolving for many of seed of woody plant species. During cryopreservation protocol to LN in plant cells take place the supercooling tissue water or vitrification, which allows the successful storage. Water content was a significant determining factor with survival of cryostored...
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