The aim of this study was to evaluate the impact of biofield energy treatment on the isotopic abundance of 13C/12C or 2H/1H or 15N/14N ≡ (PM+1)/PM in aniline; and (PM+1)/PM and 81Br/79Br ≡ (PM+2)/PM in 4-bromoaniline using Gas Chromatography-Mass Spectrometry (GC-MS). Aniline and 4-bromoaniline samples were divided into two parts: control and treated. The control part remained as untreated, while the treated part was subjected to Mr. Trivedi’s biofield energy treatment. The treated samples were subdivided in three parts named as T1, T2, and T3 for aniline and four parts named as T1, T2, T3, and T4 for 4-bromoaniline. The GC-MS data revealed that the isotopic abundance ratio of (PM+1)/PM in aniline was increased from -40.82%, 30.17% and 73.12% in T1, T2 and T3 samples respectively. However in treated samples of 4-bromoaniline the isotopic abundance ratio of PM+1/PM was increased exponentially from -4.36 % (T1) to 368.3% (T4) as compared to the control. A slight decreasing trend of the isotopic ratio of (PM+2)/PM in 4-bromoaniline was observed after biofield energy treatment. The GC-MS data suggests that the biofield energy treatment has significantly increased the isotopic abundance of 2H, 13C and 15N in the treated aniline and 4-bromoaniline, while slight decreased the isotopic abundance of 81Br in treated 4-bromoaniline as compared to their respective control.