Agro-industrial wastes were utilized to produce biohydrogen. A series of batch test were conduced at different carbon/nitrogen C/N ratios of 7, 17, 21, 31 y 46, the different mixtures were obtain by mixing crude chees whey with fruit-vegetable waste. A maximum specific biohydrogen production rate of 10.68 mmolH2/L*h and maximum biohydrogen yield of 449.84 mL H2/g COD were determined at C/N ratio of 21. The study demonstrated that co-digestion of CCW with FVW improve the biohydrogen production. These results are attributed to a better nutrient balance nutrient and the improvement of the buffer capacity of the system. These findings could be of significance in biohydrogen production cost, when biohydrogen is currently considered an expensive energy compared to other conventional energy source. The use of wastes to generate biohydrogen energy could reduce the cost, making the energy gas more available and cheaper.