Medical Science Monitor : International Medical Journal of Experimental and Clinical Research>Med Sci Monit. 25, 2019>3336–3343
Background: The serine/threonine kinase glycogen synthase kinase-3β (GSK-3β) is involved in a broad range of cellular processes, including cell proliferation, apoptosis, and inflammation. GSK-3β has been considered to play an important role in the pathogenesis of T2DM and AD, which is activated in both the periphery and central nervous system. However, the upstream and downstream factors and the underlying regulatory mechanisms of GSK-3β in T2DM and AD are unclear. Material/Methods: Here, we investigated the production of cytochrome C, Caspase-3, and Caspase-9 in in the hippocampus of DM rats and clarify the role of GSK-3β in these processes. Streptozotocin (STZ)-induced DM rats presented increased GSK-3β activity. Results: We found that cytochrome C, Caspase-3, and Caspase-9 were overproduced in the hippocampus. Furthermore, the cytochrome C, Caspase-3, and Caspase-9 levels were restored after GSK-3β inhibitors Licl treatment. Conclusions: Our results show that GSK-3β regulates the production of cytochrome C, Caspase-3, and Caspase-9 in STZ-induced rat brain and may therefore contribute to DM-caused cognitive dysfunction via inhibition of neural cell apoptosis.
Department of Neurology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China
Wagman AS, Nuss JM. Current therapies and emerging targets for the treatment of diabetes. Curr Pharm Des. 2001;7(6):417–50.
Erekat NS, Rababa'h RA, Al-Jarrah MD. Overexpression of renal proapoptotic factors is attenuated subsequent to endurance exercise in Type I diabetes: An immunohistochemistry study. Journal of Natural Science, Biology and Medicine. 2019 Jan 1;10(1):24.
Liu X, Kim CN, Yang J. Induction of apoptotic program in cell-free extracts. requirement for dATP and cytochrome c. Cell. 1996;86:147–57.
Financed by the National Centre for Research and Development under grant No. SP/I/1/77065/10 by the strategic scientific research and experimental development program:
SYNAT - “Interdisciplinary System for Interactive Scientific and Scientific-Technical Information”.