We analyse Slovak imports and exports of selected agro-food commodities between 1996 and 2005. The analysis includes the EU-15, selected new member states and candidate countries (Romania, Bulgaria), the Commonwealth of Independent States (CIS), USA and the rest of world (ROW). We show that the dynamic panel data models are appropriate tools for modelling of the agricultural trade flows. We find positive effects of the accession to the EU on Slovak agro-food export flows to the EU-15 and the other regions, while the Slovak agro-food imports show lower growth dynamic. Thus, the agricultural and food processing sectors in Slovakia have benefited from the gradual liberalization of the agro-food trade after the EU enlargement.