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The transformation of the economies of the Central and Eastern European states was on the regional level very significantly connected with the differential tendencies. In all new EU member states there has been from the beginning of the 90s to date a continuous deepening of the regional disparities on the economic level. The regional disparities result from a number of the economic, social and geographical factors. This article is a contribution to the studies of regional differentiation in the new EU member states. The goal of this study is to clear up the actual trends and major factors of regional growth and differentiation of the new EU member states.