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Nowadays, the concept of competitiveness has been become modern usage in some measure, used and accepted by broad public. Definition and explanation of this term is not simple and unambiguous as well. During the 90´s a lot of types of development zones entrenched in the Czech Republic area and became economic and social phenomenon of today. The main objective of this contribution is to analyse and assess competitiveness of development zones on the basis of regional-economic approach. The authors take advantage of both hard statistical data and information based on quality with emphasis on modern behavioural concept of classical localization theory.