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This paper addresses the problems of institutional changes in governance and the framing of biodiversity conservation policy at the level of the enlarged European Union. The theoretical basis of the paper is institutional rebuilding in Central and Eastern Europe in the context of the emerging multilevel environmental governance of the EU. The data were collected from desk study research and interviews from five Central and Eastern European countries. The results show that the emergence of multilevel governance with multiple actors' participation is prone to create tensions, but evidence from the countries studied indicates that this is not necessarily a disadvantage.