Introduction. Based on the hypothesis H that the Danube Cycling Route is the basis for the development of cycling tourism in Serbia, the research goal was to adequately demonstrate the impact that the route may have on the development of cycling tourism, using LoNGPESTEL analysis. Material and methods. The research data were collected by direct investigation, applying the survey method. The sample included 264 DBR cyclists of both genders from nine countries. Results. The research resulted in the following conclusions: that Fruška Gora Mountain (FGM) has great potential for the development of cycling tourism, and the "Danube Cycling Route" can be its backbone. Conclusions. In relation to the “Danube Cycling Route” the research has shown that one of the main advantages of FGM are: ground mobility, availability and easy access from many points on the mountain. Also, due to favourable conditions, many natural and cultural values and numerous training activities can complement the stay there.