Ultrasound-assisted matrix solid phase dispersive extraction was applied for the selective isolation and clean-up of three amphenicol antibiotics, chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FFC) from shrimp. The target antibiotics were separated on a LiChroCART-LiChrospher® 100 RP-18 (5 μm, 250 × 4 mm) analytical column in less than 9 min, with isocratic elution using a mixture of 70% ammonium acetate (0.05 M) and 30% acetonitrile (v/v). Matrix Solid Phase Dispersion protocol was optimized in terms of extraction sorbent and elution solvent. Two polymer based (Oasis and Nexus) sorbents and one silica based (Lichrolut C18) were compared and different elution solvents such as methanol, acetone, acetonitrile and isopropanol were evaluated based on the achieved recovery rates as well as on the cleanup efficiency. The extraction procedure was performed with and without sonication to evaluate the impact of ultrasounds. TAP and FFC were monitored at 234 nm and CAP at 280 nm by a photodiode array detector. The method was validated according to the European Union Decision 2002/657/EC in terms of linearity, selectivity, stability, accuracy, precision and sensitivity. Detection capability values (CCb) were 64.6 μg/kg for TAP and 1046.8 μg/kg for FFC and 63.8 μg/ kg for CAP.
 Commission Regulation (EU) No 37/2010 of 22 December 2009,
L.15/1-72, on pharmacologically active substances and their
classification regarding maximum residue limits in foodstuffs
of animal origin
 Commission Decision (EU) 181/2003, L71/17 of 13 March 2003,
amending Decision 2002/657/EC as regards the setting of
minimum required performance limits
(MRPLs) for certain residues in food of animal origin (notified
under document number C(2003) 764).
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SYNAT - “Interdisciplinary System for Interactive Scientific and Scientific-Technical Information”.