Unravelling peptidomes by in silico mining

Peptides of great number and diversity occur in all domains of life and exhibit a range of pharmaceutically relevant bioactivities. The complexity of biological samples including human cells or tissues, plant extracts or animal venom cocktails, often impedes the discovery of novel bioactive peptides using mass spectrometrybased peptidomics analysis. An increasing number of publicly available genome and transcriptome datasets, together with refined bioinformatics analysis, allows for rapid identification of novel peptides which may have been previously unrecognized. Moreover, a combination of information extracted from in silico mining approaches together with data derived from mass spectrometrybased studies provides new impetus for future peptidome analyses, including the discovery of novel bioactive peptides that can serve as starting points for drug development.