An analysis of the fractions of free acids and phenolic acids liberated by way of hydrolysis in the rhizomes and herbaceous tissues of Sanguisorba officinalis L. was conducted through utilizing the 2D-TLC method. Fifteen phenolic acids were identified. Our work showed that ellagic, protocatechuic, gentisic, p-hydroxybenzoic, syringic, vannilic and ferulic acids were common in all tested fractions, and gallic and protocatechuic acids were dominant in fractions obtained from the rhizomes, while caffeic, p-coumaric, syringic, vannilic and ferulic acids were abundant in the herbaceous tissues. However, α and β - resorcylic acids were detected only in rhizome phenolic acid fractions liberated from their conjunction with sugars and alcohols. Furthermore, chlorogenic acid was present only in a free form in the herbaceous tissues, while p-hydroxyphenylacetic acid was liberated from conjunction from a herbaceous extract.
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