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In this paper we describe the application of two-photon microscopy (2PM) to the study of meiosis in plants. Fresh, unfixed anthers of Agapanthus umbelatus were briefly incubated on a minimal medium containing the DNA fluorophore DAPI. DAPI incorporation took place in about 30min and nuclei and other DNA-containing organelles kept their fluorescence for more than 24h. Using 2PM it was possible to optically...
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