Search results for: Dmitri B. Papkovsky

Items from 1 to 20 out of 76 results

article

A sensor‐based system for rapid on‐site testing of microbial contamination in meat samples and carcasses

Elisa Santovito, Sophia Elisseeva, Conor Smyth, Malco Cruz‐Romero, more

Journal of Applied Microbiology > 132 > 2 > 1210 - 1220

Aims To develop an oxygen sensor‐based method for testing total aerobic viable counts (TVC) in raw meat samples and cattle carcass swabs, which is rapid, simple, affordable, provides good sensitivity and analytical performance and allows on‐site use. Methods and Results The test uses the same sample preparation procedure as the established plate counting TVC method for meat samples and carcasses,...

article

Estimation of the Mitochondrial Membrane Potential Using Fluorescence Lifetime Imaging Microscopy

Irina A. Okkelman, Dmitri B. Papkovsky, Ruslan I. Dmitriev

Cytometry Part A > 97 > 5 > 471 - 482

Monitoring of cell metabolism represents an important application area for fluorescence lifetime imaging microscopy (FLIM). In particular, assessment of mitochondrial membrane potential (MMP) in complex three‐dimensional multicellular in vitro, ex vivo, and in vivo models would enable improved segmentation and functional discrimination of cell types, directly report on the mitochondrial function and...

article

Imaging of oxygen and hypoxia in cell and tissue samples

Dmitri B. Papkovsky, Ruslan I. Dmitriev

Cellular and Molecular Life Sciences > 2018 > 75 > 16 > 2963-2980

Molecular oxygen (O₂) is a key player in cell mitochondrial function, redox balance and oxidative stress, normal tissue function and many common disease states. Various chemical, physical and biological methods have been proposed for measurement, real-time monitoring and imaging of O₂ concentration, state of decreased O₂ (hypoxia) and related parameters in cells and tissue. Here, we review the established...

article

Nanoparticle‐Based Fluoroionophore for Analysis of Potassium Ion Dynamics in 3D Tissue Models and In Vivo

Bernhard J. Müller, Alexander V. Zhdanov, Sergey M. Borisov, Tara Foley, more

Advanced Functional Materials > 28 > 9 > n/a - n/a

The imaging of real‐time fluxes of K⁺ ions in live cell with high dynamic range (5–150 × 10⁻³m) is of paramount importance for neuroscience and physiology of the gastrointestinal tract, kidney, and other tissues. In particular, the research on high‐performance deep‐red fluorescent nanoparticle‐based biosensors is highly anticipated. It is found that boron‐dipyrromethene (BODIPY)‐based K⁺‐sensitive...

article

Live cell imaging of mouse intestinal organoids reveals heterogeneity in their oxygenation

Irina A. Okkelman, Tara Foley, Dmitri B. Papkovsky, Ruslan I. Dmitriev

Biomaterials > 2017 > 146 > C > 86-96

Intestinal organoids are widely applied in stem cell research, regenerative medicine, toxicology, pharmacology, and host-microbe interactions research. The variability of oxidative metabolism for stem and differentiated cell types constituting organoid is known to be important but so far it has not been studied in details. Here, we report the use of live cell microscopy of oxygen via the phosphorescence...

article

Free radical production and detoxification in complex IV deficient cancer cells

Alexander V. Zhdanov, Dmitry E. Andreev, Pavel V. Baranov, Dmitri B. Papkovsky

Free Radical Biology and Medicine > 2017 > 108 > S1 > S104

Mitochondrial membrane potential (DYm) is one of the key drivers of free radical production. Changes in DYm strongly affect free radical turnover in both mitochondria and cytosol. We found that in cytochrome c oxidase deficient HCT116 cells F1Fo ATP synthase (mATPase) is reversed in order to maintain DYm polarisation. For small energy costs, mATPase enables mitochondrial protein import, Ca²⁺ turnover...

article

Low energy costs of F1Fo ATP synthase reversal in colon carcinoma cells deficient in mitochondrial complex IV

Alexander V. Zhdanov, Dmitry E. Andreev, Pavel V. Baranov, Dmitri B. Papkovsky

Free Radical Biology and Medicine > 2017 > 106 > C > 184-195

Mitochondrial polarisation is paramount for a variety of cellular functions. Under ischemia, mitochondrial membrane potential (ΔΨm) and proton gradient (ΔpH) are maintained via a reversal of mitochondrial F1Fo ATP synthase (mATPase), which can rapidly deplete ATP and drive cells into energy crisis. We found that under normal conditions in cells with disassembled cytochrome c oxidase complex (COX-deficient...

article

Planar implantable sensor for in vivo measurement of cellular oxygen metabolism in brain tissue

Vassiliy Tsytsarev, Fatih Akkentli, Elena Pumbo, Qinggong Tang, more

Journal of Neuroscience Methods > 2017 > 281 > C > 1-6

Brain imaging methods are continually improving. Imaging of the cerebral cortex is widely used in both animal experiments and charting human brain function in health and disease. Among the animal models, the rodent cerebral cortex has been widely used because of patterned neural representation of the whiskers on the snout and relative ease of activating cortical tissue with whisker stimulation.We...

article

Cellular ROS imaging with hydro-Cy3 dye is strongly influenced by mitochondrial membrane potential

Alexander V. Zhdanov, Gabriella Aviello, Ulla G. Knaus, Dmitri B. Papkovsky

Biochimica et Biophysica Acta (BBA) - General Subjects > 2017 > 1861 > 2 > 198-204

Hydrocyanines are widely used as fluorogenic probes to monitor reactive oxygen species (ROS) generation in cells. Their brightness, stability to autoxidation and photobleaching, large signal change upon oxidation, pH independence and red/near infrared emission are particularly attractive for imaging ROS in live tissue.Using confocal fluorescence microscopy we have examined an interference of mitochondrial...

article

Phosphorescence based O2 sensors – Essential tools for monitoring cell and tissue oxygenation and its impact on metabolism

Dmitri B. Papkovsky, Alexander V. Zhdanov

Free Radical Biology and Medicine > 2016 > 101 > C > 202-210

Oxygenation condition at the cellular level is a critical factor in tissue physiology and common pathophysiological states including cancer, metabolic disorders, ischemia-reperfusion injury and inflammation. O₂ and ROS signalling and hypoxia research are rapidly growing areas spanning life and biomedical sciences, but still many current cell and tissue models and experimental set ups lack physiological...

article

In Situ Control of Cell and Tissue Oxygenation with Phosphorescence Based O2 Sensors and Imaging Probes

Dmitri B. Papkovsky, Alexander B Zhdanov, Ruslan B Dmitriev

Free Radical Biology and Medicine > 2016 > 100 > S > S186

Hypoxia and reactive oxygen species (ROS) signalling research is a rapidly growing area spanning life and biomedical sciences. Still many current in vitro models and experimental set ups lack physiological relevance, particularly precise in situ control of cell and tissue O₂. Quenched-phosphorescence O₂ sensing enables implementation of such control, replication of physiological conditions in respiring...

article

Quantitative analysis of mucosal oxygenation using ex vivo imaging of healthy and inflamed mammalian colon tissue

Alexander V. Zhdanov, Irina A. Okkelman, Anna V. Golubeva, Barbara Doerr, more

Cellular and Molecular Life Sciences > 2017 > 74 > 1 > 141-151

Colonic inflammation is associated with decreased tissue oxygenation, significantly affecting gut homeostasis. However, the crosstalk between O₂ consumption and supply in the inflamed tissue are not fully understood. Using a murine model of colitis, we analysed O₂ in freshly prepared samples of healthy and inflamed colon tissue. We developed protocols for efficient ex vivo staining of mouse distal...

article

Phosphorescent oxygen sensors produced from polyolefin fibres by solvent-crazing method

Swagata Banerjee, Olga V. Arzhakova, Alla A. Dolgova, Dmitri B. Papkovsky

Sensors and Actuators B: Chemical > 2016 > 230 > C > 434-441

Development of phosphorescent oxygen sensing materials based on commercially available semicrystalline polymer fibres (polyolefins, PP and PE) is described. The Pt-benzoporphyrin dye was incorporated into the nanoporous network of polyolefin fibres formed upon tensile drawing in the presence of physically active liquid environment via the mechanism of solvent crazing. Optimised fibre sensors showed...

article

High throughput non-destructive assessment of quality and safety of packaged food products using phosphorescent oxygen sensors

Swagata Banerjee, Caroline Kelly, Joseph P. Kerry, Dmitri B. Papkovsky

Trends in Food Science & Technology > 2016 > 50 > C > 85-102

Intelligent and active packaging technologies have gained attention in recent time due to increased demand by the consumers and manufacturers for sustaining the quality and safety of food products, improved shelf-life as well as real time monitoring of the packaging, storage and handling processes. In this context, phosphorescence based sensors for molecular oxygen (O₂) are important tools for monitoring...

article

High-Resolution Analysis of Molecular Oxygen in Mammalian Cell Models by Phosphorescence Lifetime Imaging Microscopy

Ruslan I. Dmitriev, James Jenkins, Irina A. Okkelman, Dmitri B. Papkovsky

Biophysical Journal > 2016 > 110 > 3 Supplement 1 > 518a-519a

article

Application of phosphorescent oxygen sensors in in-package dielectric barrier discharge plasma environment

S.K. Pankaj, Caroline A. Kelly, C. Bueno-Ferrer, Joe P. Kerry, more

Innovative Food Science & Emerging Technologies > 2016 > 33 > C > 234-239

Dielectric barrier discharge plasma treatment of two different types of phosphorescent oxygen sensors under different in-package gas mixture has been studied. Commercial Optech™-O₂ platinum and microporous polypropylene (PP) membrane sensors impregnated with platinum benzoporphyrin (PtBP) dye were found effective for oxygen sensing when DBD plasma treated under modified atmospheric conditions. When...

article

A novel effect of DMOG on cell metabolism: direct inhibition of mitochondrial function precedes HIF target gene expression

Alexander V. Zhdanov, Irina A. Okkelman, Fergus W.J. Collins, Silvia Melgar, more

Biochimica et Biophysica Acta (BBA) - Bioenergetics > 2015 > 1847 > 10 > 1254-1266

Abnormal accumulation of oncometabolite fumarate and succinate is associated with inhibition of mitochondrial function and carcinogenesis. By competing with α-ketoglutarate, oncometabolites also activate hypoxia inducible factors (HIFs), which makes oncometabolite mimetics broadly utilised in hypoxia research. We found that dimethyloxalylglycine (DMOG), a synthetic analogue of α-ketoglutarate, commonly...

article

Solid-state oxygen sensors based on phosphorescent diiodo-borondipyrromethene dye

Swagata Banerjee, Rimma T. Kuznetsova, Dmitri B. Papkovsky

Sensors & Actuators: B. Chemical > 2015 > 212 > Complete > 229-234

The detailed study of oxygen sensitive materials comprising diiodo-borondipyrromethene dye (I ₂ -BODIPY) encapsulated in hydrophobic polymers (Polysulfone, polyphenylene sulphide and fibrous polypropylene) is presented. These materials display strong absorption in the visible region and moderate room temperature phosphorescence in the near infrared region. They exhibit high sensitivity towards...

article

Oxygen-sensing scaffolds for 3-dimensional cell and tissue culture

James Jenkins, Ruslan I. Dmitriev, Karl Morten, Kieran W. McDermott, more

Acta Biomaterialia > 2015 > 16 > Complete > 126-135

Porous membrane scaffolds are widely used materials for three-dimensional cell cultures and tissue models. Additional functional modification of such scaffolds can significantly extend their use and operational performance. Here we describe hybrid microporous polystyrene-based scaffolds impregnated with a phosphorescent O ₂ -sensitive dye PtTFPP, optimized for live cell fluorescence microscopy...

article

Differential contribution of key metabolic substrates and cellular oxygen in HIF signalling

Alexander V. Zhdanov, Alicia H.C. Waters, Anna V. Golubeva, Dmitri B. Papkovsky

Experimental Cell Research > 2015 > 330 > 1 > 13-28

Changes in availability and utilisation of O ₂ and metabolic substrates are common in ischemia and cancer. We examined effects of substrate deprivation on HIF signalling in PC12 cells exposed to different atmospheric O ₂ . Upon 2–4h moderate hypoxia, HIF-α protein levels were dictated by the availability of glutamine and glucose, essential for deep cell deoxygenation and glycolytic...

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INFONA - science communication portal

Search results for: Dmitri B. Papkovsky

A sensor‐based system for rapid on‐site testing of microbial contamination in meat samples and carcasses

Estimation of the Mitochondrial Membrane Potential Using Fluorescence Lifetime Imaging Microscopy

Imaging of oxygen and hypoxia in cell and tissue samples

Nanoparticle‐Based Fluoroionophore for Analysis of Potassium Ion Dynamics in 3D Tissue Models and In Vivo

Live cell imaging of mouse intestinal organoids reveals heterogeneity in their oxygenation

Free radical production and detoxification in complex IV deficient cancer cells

Low energy costs of F1Fo ATP synthase reversal in colon carcinoma cells deficient in mitochondrial complex IV

Planar implantable sensor for in vivo measurement of cellular oxygen metabolism in brain tissue

Cellular ROS imaging with hydro-Cy3 dye is strongly influenced by mitochondrial membrane potential

Phosphorescence based O2 sensors – Essential tools for monitoring cell and tissue oxygenation and its impact on metabolism

In Situ Control of Cell and Tissue Oxygenation with Phosphorescence Based O2 Sensors and Imaging Probes

Quantitative analysis of mucosal oxygenation using ex vivo imaging of healthy and inflamed mammalian colon tissue

Phosphorescent oxygen sensors produced from polyolefin fibres by solvent-crazing method

High throughput non-destructive assessment of quality and safety of packaged food products using phosphorescent oxygen sensors

High-Resolution Analysis of Molecular Oxygen in Mammalian Cell Models by Phosphorescence Lifetime Imaging Microscopy

Application of phosphorescent oxygen sensors in in-package dielectric barrier discharge plasma environment

A novel effect of DMOG on cell metabolism: direct inhibition of mitochondrial function precedes HIF target gene expression

Solid-state oxygen sensors based on phosphorescent diiodo-borondipyrromethene dye

Oxygen-sensing scaffolds for 3-dimensional cell and tissue culture

Differential contribution of key metabolic substrates and cellular oxygen in HIF signalling

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