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A detailed understanding of subcellular structure motility is critical to understanding how cells regulate the delivery of specific proteins from the site of synthesis to the site of action. Diverse modalities have been observed for subcellular dynamics, such as directional movement, random movement, tethered movement, object appearing, and disappearing. Motion modality detection is important for...
High-resolution MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight) mass spectrometry has shown promise as a screening tool for detecting discriminatory protein patterns. The major computational obstacle in analyzing MALDI-TOF data is a large number of mass/charge peaks (a.k.a. features, data points). With the number of data points easily going beyond one million for a single sample,...
Understanding the motility of subcellular particles like organelles, vesicles, or mRNAs is critical to understand how cells regulate delivery of specific proteins from the site of synthesis to the site of action. The goal of this paper is to present a framework of feature selection, matching, and evaluation for the segmentation and tracking of green fluorescent protein (GFP) labeled subcellular structures...
Tagging and tracking protein molecules with the help of laser scanning confocal microscope (LSCM) is a key to better understanding of proteomics in diverse aspects. One challenge of tracking multiple green fluorescent protein (GFP) clusters is how to deal with the interaction between multiple objects, namely splitting and merging. In this paper, we propose a framework to track multiple GFP clusters...
Tagging and tracking protein compounds/compounds are key to a better understanding of proteomics such as protein-protein interaction and protein signaling pathway. In this paper, a generalized region tracking framework by statistical particle filter (PF) is presented for tracing the movement of protein compounds in confocal microscopy images. To effectively select the features to be tracked, a grid-based...
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