Search results for: John E. P. Syka

Items from 1 to 14 out of 14 results

article

Infrared Multiphoton Dissociation Enables Top‐Down Characterization of Membrane Protein Complexes and G Protein‐Coupled Receptors

Corinne A. Lutomski, Tarick J. El‐Baba, Joshua D. Hinkle, Idlir Liko, more

Angewandte Chemie International Edition > 62 > 36 > n/a - n/a

Membrane proteins are challenging to analyze by native mass spectrometry (MS) as their hydrophobic nature typically requires stabilization in detergent micelles that are removed prior to analysis via collisional activation. There is however a practical limit to the amount of energy which can be applied, which often precludes subsequent characterization by top‐down MS. To overcome this barrier, we...

article

Infrared Multiphoton Dissociation Enables Top‐Down Characterization of Membrane Protein Complexes and G Protein‐Coupled Receptors

Corinne A. Lutomski, Tarick J. El‐Baba, Joshua D. Hinkle, Idlir Liko, more

Angewandte Chemie > 135 > 36 > n/a - n/a

article

Ion-Ion Proton Transfer and Parallel Ion Parking for the Analysis of Mixtures of Intact Proteins on a Modified Orbitrap Mass Analyzer

Scott A. Ugrin, A. Michelle English, John E. P. Syka, Dina L. Bai, more

Journal of The American Society for Mass Spectrometry > 2019 > 30 > 10 > 2163-2173

We have enabled parallel ion parking on a modified Orbitrap Elite™ as a way to control ion-ion proton transfer reactions via selective activation of a range of ions. The result is the concentration of the majority of ion current from multiple charge states of each precursor proteoform into a single charge state, maximizing signal intensity and increasing effective sensitivity compared to conventional...

article

Front-End Electron Transfer Dissociation Coupled to a 21 Tesla FT-ICR Mass Spectrometer for Intact Protein Sequence Analysis

Chad R. Weisbrod, Nathan K. Kaiser, John E. P. Syka, Lee Early, more

Journal of The American Society for Mass Spectrometry > 2017 > 28 > 9 > 1787-1795

High resolution mass spectrometry is a key technology for in-depth protein characterization. High-field Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) enables high-level interrogation of intact proteins in the most detail to date. However, an appropriate complement of fragmentation technologies must be paired with FTMS to provide comprehensive sequence coverage, as well as...

article

Sulfur Pentafluoride is a Preferred Reagent Cation for Negative Electron Transfer Dissociation

Matthew J. P. Rush, Nicholas M. Riley, Michael S. Westphall, John E. P. Syka, more

Journal of The American Society for Mass Spectrometry > 2017 > 28 > 7 > 1324-1332

Negative mode proteome analysis offers access to unique portions of the proteome and several acidic post-translational modifications; however, traditional collision-based fragmentation methods fail to reliably provide sequence information for peptide anions. Negative electron transfer dissociation (NETD), on the other hand, can sequence precursor anions in a high-throughput manner. Similar to other...

article

Enhanced Dissociation of Intact Proteins with High Capacity Electron Transfer Dissociation

Nicholas M. Riley, Christopher Mullen, Chad R. Weisbrod, Seema Sharma, more

Journal of The American Society for Mass Spectrometry > 2016 > 27 > 3 > 520-531

Electron transfer dissociation (ETD) is a valuable tool for protein sequence analysis, especially for the fragmentation of intact proteins. However, low product ion signal-to-noise often requires some degree of signal averaging to achieve high quality MS/MS spectra of intact proteins. Here we describe a new implementation of ETD on the newest generation of quadrupole-Orbitrap-linear ion trap Tribrid,...

article

Activated Ion ETD Performed in a Modified Collision Cell on a Hybrid QLT-Oribtrap Mass Spectrometer

Aaron R. Ledvina, Christopher M. Rose, Graeme C. McAlister, John E. P. Syka, more

Journal of The American Society for Mass Spectrometry > 2013 > 24 > 11 > 1623-1633

We describe the implementation and characterization of activated ion electron transfer dissociation (AI-ETD) on a hybrid QLT-Orbitrap mass spectrometer. AI-ETD was performed using a collision cell that was modified to enable ETD reactions, in addition to normal collisional activation. The instrument manifold was modified to enable irradiation of ions along the axis of this modified cell with IR photons...

article

Multipurpose Dissociation Cell for Enhanced ETD of Intact Protein Species

Christopher M. Rose, Jason D. Russell, Aaron R. Ledvina, Graeme C. McAlister, more

Journal of The American Society for Mass Spectrometry > 2013 > 24 > 6 > 816-827

We describe and characterize an improved implementation of ETD on a modified hybrid linear ion trap-Orbitrap instrument. Instead of performing ETD in the mass-analyzing quadrupole linear ion trap (A-QLT), the instrument collision cell was modified to enable ETD. We partitioned the collision cell into a multi-section rf ion storage and transfer device to enable injection and simultaneous separate storage...

article

Activated-Ion Electron Transfer Dissociation Improves the Ability of Electron Transfer Dissociation to Identify Peptides in a Complex Mixture

Aaron R. Ledvina, Nicole A. Beauchene, Graeme C. McAlister, John E. P. Syka, more

Analytical Chemistry > 2010 > 82 > 24 > 10068 - 10074

article

A Proteomics Grade Electron Transfer Dissociation-Enabled Hybrid Linear Ion Trap-Orbitrap Mass Spectrometer

Graeme C. McAlister, W. Travis Berggren, Jens Griep-Raming, Stevan Horning, more

Journal of Proteome Research > 2008 > 7 > 8 > 3127 - 3136

article

Supplemental Activation Method for High-Efficiency Electron-Transfer Dissociation of Doubly Protonated Peptide Precursors

Danielle L. Swaney, Graeme C. McAlister, Matthew Wirtala, Jae C. Schwartz, more

Analytical Chemistry > 2007 > 79 > 2 > 477 - 485

article

Electron transfer dissociation of peptide anions

Joshua J. Coon, Jeffrey Shabanowitz, Donald F. Hunt, John E. P. Syka

Journal of The American Society for Mass Spectrometry > 2005 > 16 > 6 > 880-882

Ion/ion reactions of multiply deprotonated peptide anions with xenon radical cations result in electron abstraction to generate charge-reduced peptide anions containing a free-radical site. Peptide backbone cleavage then occurs by hydrogen radical abstraction from a backbone amide N to facilitate cleavage of the adjacent C-C bond, thereby producing a- and x-type product ions. Introduction of free-radical...

article

Novel Linear Quadrupole Ion Trap/FT Mass Spectrometer: Performance Characterization and Use in the Comparative Analysis of Histone H3 Post-translational Modifications

John E. P. Syka, Jarrod A. Marto, Dina L. Bai, Stevan Horning, more

Journal of Proteome Research > 2004 > 3 > 3 > 621 - 626

article

A two-dimensional quadrupole ion trap mass spectrometer

Jae C. Schwartz, Michael W. Senko, John E. P. Syka

Journal of The American Society for Mass Spectrometry > 2002 > 13 > 6 > 659-669

The use of a linear or two-dimensional (2-D) quadrupole ion trap as a high performance mass spectrometer is demonstrated. Mass analysis is performed by ejecting ions out a slot in one of the rods using the mass selective instability mode of operation. Resonance ejection and excitation are utilized to enhance mass analysis and to allow isolation and activation of ions for MSⁿ capability. Improved trapping...

Filter options

Publication date

Last 3 years (2)

Close

Date range setting

Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.

From:

To:
Set your own date range

INFONA - science communication portal

Search results for: John E. P. Syka

Add recipient

Sending message cancelled

Are you sure you want to cancel sending this message?

Send message

Filter options

Publication date

Date range setting

Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.

Keywords

Data set

Journal

Reporting an error / abuse

Sending the report failed

Accessibility options