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The effectiveness of any proteomics database search depends on the theoretical candidate information contained in the protein database. Unfortunately, candidate entries from protein databases such as UniProt rarely contain all the post‐translational modifications (PTMs), disulfide bonds, or endogenous cleavages of interest to researchers. These omissions can limit discovery of novel and biologically...
Pilot Project #1—the identification and characterization of human histone H4 proteoforms by top‐down MS—is the first project launched by the Consortium for Top‐Down Proteomics (CTDP) to refine and validate top‐down MS. Within the initial results from seven participating laboratories, all reported the probability‐based identification of human histone H4 (UniProt accession P62805) with expectation values...
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