Search results for: Jiban K. Dattagupta

Items from 1 to 7 out of 7 results

article

Role of remote scaffolding residues in the inhibitory loop pre-organization, flexibility, rigidification and enzyme inhibition of serine protease inhibitors

Sudip Majumder, Susmita Khamrui, Jhimli Dasgupta, Jiban K. Dattagupta, more

BBA - Proteins and Proteomics > 2012 > 1824 > 7 > 882-890

Canonical serine protease inhibitors interact with cognate enzymes through the P3–P2′ region of the inhibitory loop while its scaffold hardly makes any contact. Neighboring scaffolding residues like Arginines or Asparagine shape-up the inhibitory loop and favor the resynthesis of cleaved scissile bond. However, role of remote scaffolding residues, which are not involved in religation, was not properly...

article

C‐Terminal extension of a plant cysteine protease modulates proteolytic activity through a partial inhibitory mechanism

Sruti Dutta, Debi Choudhury, Jiban K. Dattagupta, Sampa Biswas

The FEBS Journal > 278 > 17 > 3012 - 3024

The amino acid sequence of ervatamin‐C, a thermostable cysteine protease from a tropical plant, revealed an additional 24‐amino‐acid extension at its C‐terminus (CT). The role of this extension peptide in zymogen activation, catalytic activity, folding and stability of the protease is reported. For this study, we expressed two recombinant forms of the protease in Escherichia coli, one retaining the...

article

A thermostable cysteine protease precursor from a tropical plant contains an unusual C-terminal propeptide: cDNA cloning, sequence comparison and molecular modeling studies

Raka Ghosh, Jiban K. Dattagupta, Sampa Biswas

Biochemical and Biophysical Research Communications > 2007 > 362 > 4 > 965-970

We report here the cloning and characterization of the entire cDNA of a papain-like cysteine protease from a tropical flowering plant. The 1098-bp ORF of the cDNA codify a protease precursor having a signal peptide of 19 amino acids, a cathepsin-L like N-terminal proregion of 114 amino acids, a mature enzyme part of 208 amino acids and a C-terminal proregion of 24 amino acids. The derived amino acid...

article

Spacer Asn Determines the Fate of Kunitz (STI) Inhibitors, as Revealed by Structural and Biochemical Studies on WCI Mutants ^‡

Jhimli Dasgupta, Susmita Khamrui, Jiban K. Dattagupta, Udayaditya Sen

Biochemistry > 2006 > 45 > 22 > 6783 - 6792

article

Single mutation at P1 of a chymotrypsin inhibitor changes it to a trypsin inhibitor: X-ray structural (2.15 Å) and biochemical basis

Susmita Khamrui, Jhimli Dasgupta, Jiban K. Dattagupta, Udayaditya Sen

BBA - Proteins and Proteomics > 2005 > 1752 > 1 > 65-72

Change in specificity, caused by the mutations at P1 site, of the serine protease inhibitors of different families is reported in the literature, but Kunitz (STI) family inhibitors are almost unexplored in this regard. In this paper, we present the crystal structure of a P1 variant of winged bean chymotrypsin inhibitor (WCI) belonging to Kunitz (STI) family, supplemented by biochemical, phylogenetic...

article

Crystal Structures of HbA ₂ and HbE and Modeling of Hemoglobin δ ₄ : Interpretation of the Thermal Stability and the Antisickling Effect of HbA ₂ and Identification of the Ferrocyanide Binding Site in Hb ^‡

Udayaditya Sen, Jhimli Dasgupta, Debi Choudhury, Poppy Datta, more

Biochemistry > 2004 > 43 > 39 > 12477 - 12488

article

Structural Basis of the Unusual Stability and Substrate Specificity of Ervatamin C, a Plant Cysteine Protease from Ervatamia coronaria ^‡

Piyali Guha Thakurta, Sampa Biswas, Chandana Chakrabarti, Monica Sundd, more

Biochemistry > 2004 > 43 > 6 > 1532 - 1540

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Search results for: Jiban K. Dattagupta

Role of remote scaffolding residues in the inhibitory loop pre-organization, flexibility, rigidification and enzyme inhibition of serine protease inhibitors

C‐Terminal extension of a plant cysteine protease modulates proteolytic activity through a partial inhibitory mechanism

A thermostable cysteine protease precursor from a tropical plant contains an unusual C-terminal propeptide: cDNA cloning, sequence comparison and molecular modeling studies

Spacer Asn Determines the Fate of Kunitz (STI) Inhibitors, as Revealed by Structural and Biochemical Studies on WCI Mutants ‡

Single mutation at P1 of a chymotrypsin inhibitor changes it to a trypsin inhibitor: X-ray structural (2.15 Å) and biochemical basis

Crystal Structures of HbA 2 and HbE and Modeling of Hemoglobin δ 4 : Interpretation of the Thermal Stability and the Antisickling Effect of HbA 2 and Identification of the Ferrocyanide Binding Site in Hb ‡

Structural Basis of the Unusual Stability and Substrate Specificity of Ervatamin C, a Plant Cysteine Protease from Ervatamia coronaria ‡

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Spacer Asn Determines the Fate of Kunitz (STI) Inhibitors, as Revealed by Structural and Biochemical Studies on WCI Mutants ^‡

Crystal Structures of HbA ₂ and HbE and Modeling of Hemoglobin δ ₄ : Interpretation of the Thermal Stability and the Antisickling Effect of HbA ₂ and Identification of the Ferrocyanide Binding Site in Hb ^‡

Structural Basis of the Unusual Stability and Substrate Specificity of Ervatamin C, a Plant Cysteine Protease from Ervatamia coronaria ^‡