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In this study, we sought to improve the hydrolytic activity of a His4-type single finger domain (f2), which was previously derived from the second finger domain (f2′) of the Sp1 zinc finger protein (Sp1wt), which has 3 tandem finger domains (f1′, f2′, and f3′). To this end, 2 His4-type single finger domains were generated by mutating 2 Cys residues participating in Zn(II) coordination with the His...
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