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4-Hydroxyphenylacetic acid (4-HPA) can inhibit monophenolase (IC50=0.79 mM) and activate diphenolase of mushroom tyrosinase with its increasing concentrations. The activation kinetics, analyzed by Lineweaver-Burk plots, indicated 4-HPA to be a competitive and uncompetitive mixed-type activator of diphenolase when L-DOPA was used as substrate. 4-HPA can not only inhibit the oxy form but also activate...
This study demonstrates that Aminoethylisothiourea (AET), a potent inhibitor of inducible nitric oxide synthase (NOS), is an irreversible competitive inhibitor of the diphenolase activity of mushroom tyrosinase when L-DOPA was assayed spectrophotometrically in vitro. The inhibition of diphenolase activity of tyrosinase by AET showed positive kinetic cooperativity which arose from the protection of...
Papain (EC 3.4.22.2) has been chemically modified using two reagents including different anhydrides of maleic and poly-maleic acids. The average ratio of modified-NH2 was tested by trinitrobenzenesulfonic acid (TNBS) method. The native and modified papain were purified by dialysis and their structures were characterized by FTIR. The factor related with the activity of the modified papain, such as...
Nylon membranes are first hydrolyed with dilute HCl and then treated with chitosan before being used as the affinity carrier. Papain as a ligand has been immobilized on the activated membranes with glutaraldehyde as a crosslinking agent. The factors involving with the activity of immobilized papain, such as concentration of glutaraldehyde, pH, temperature, reaction time, and the amount of added papain...
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