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This paper describes the immobilization and quantification of 15base ssDNA on 250 nm silica nanoparticles. 1 up to 2400 amine functionalized ssDNA are coupled to a carboxyl functionalized nanoparticle using EDC/NHS chemistry. The amount of ssDNA on the nanoparticles is quantified by bulk fluorescence measurements in a microtiterplate reader on both the nanoparticles in dense solution and the supernatant...
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