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AimsTo develop an effective multiplex polymerase chain reaction (PCR) for the simultaneous detection of three important Vibrio species, Vibrio cholerae (Vc), V. parahaemolyticus (Vp) and V. vulnificus (Vv) using the groEL gene, a potential phylogenetic marker.
Methods and ResultsThree species‐specific primer sets were designed to target Vc, Vp and Vv. A total of 131 Vibrio and non‐Vibrio strains...
Aims: Vibrio parahaemolyticus is a significant cause of human gastrointestinal disorders and is transmitted through ingestion of raw or undercooked contaminated seafood. We used the groEL gene for the species‐specific detection of V. parahaemolyticus from artificially inoculated shellfish, fish and seawater.
Methods and Results: The nucleotide sequences of 24 Vibrio and seven non‐Vibrio spp. were...
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