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A simple graphical method for the determination of reversible inhibition type, inhibition constant (Ki) and estimation of fifty percent of inhibition I50 of an enzyme reaction is described. The method consists of plotting experimental data as “degree of inhibition” versus the inhibitor concentration at two or more concentrations of substrate. Diagnosis of inhibition type is based on determination...
Analytical technology based on sensors is an extremely broad field which impacts on many major industrial sectors such as the pharmaceutical, healthcare, food, and agriculture industries as well as environmental monitoring. This review will highlight the research carried out during the last 5 years on biosensors that are based on enzyme inhibition for determination of pollutants and toxic compounds...
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