Search results for: Kumiko Yoshizawa-Kumagaye

Items from 1 to 14 out of 14 results

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Easy‐to‐Attach/Detach Solubilizing‐Tag‐Aided Chemical Synthesis of an Aggregative Capsid Protein

Shugo Tsuda, Masayoshi Mochizuki, Hiroyuki Ishiba, Kumiko Yoshizawa‐Kumagaye, more

Angewandte Chemie > 130 > 8 > 2127 - 2131

A solubilizing Trt‐K₁₀ tag was developed for the effective chemical preparation of peptides/proteins with low solubility. The Trt‐K₁₀ tag comprises a hydrophilic oligo‐Lys sequence and a trityl anchor, and can be selectively introduced to a side chain thiol of Cys of deprotected peptides/proteins with a trityl alcohol‐type introducing reagent Trt(OH)‐K₁₀ under acidic conditions. Significantly, the...

article

Easy‐to‐Attach/Detach Solubilizing‐Tag‐Aided Chemical Synthesis of an Aggregative Capsid Protein

Shugo Tsuda, Masayoshi Mochizuki, Hiroyuki Ishiba, Kumiko Yoshizawa‐Kumagaye, more

Angewandte Chemie International Edition > 57 > 8 > 2105 - 2109

article

Non-pretreated O-acyl isopeptide of amyloid β peptide 1–42 is monomeric with a random coil structure but starts to aggregate in a concentration-dependent manner

Taku Yoshiya, Takahiro Maruno, Tsuyoshi Uemura, Shigeru Kubo, more

Bioorganic & Medicinal Chemistry Letters > 2014 > 24 > 16 > 3861-3864

An isopeptide of amyloid β peptide 1–42 (isoAβ42) was considered as a non-aggregative precursor molecule for the highly aggregative Aβ42. It has been applied to biological studies after several pretreatments. Here we report that isoAβ42 is monomeric with a random coil structure at 40μM without any pretreatment. But we also found that isoAβ42 retains a slight aggregative nature, which is significantly...

article

O‐Acyl isopeptide method: development of an O‐acyl isodipeptide unit for Boc SPPS and its application to the synthesis of Aβ1‐42 isopeptide

Taku Yoshiya, Tsuyoshi Uemura, Takahiro Maruno, Shigeru Kubo, more

Journal of Peptide Science > 20 > 9 > 669 - 674

The O‐acyl isopeptide method was developed for the efficient preparation of difficult sequence‐containing peptide. Furthermore, development of the O‐acyl isodipeptide unit for Fmoc chemistry simplified its synthetic procedure by solid‐phase peptide synthesis. Here, we report a novel isodipeptide unit for Boc chemistry, and the unit was successfully applied to the synthesis of amyloid β peptide. Combination...

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Biological properties of adrenomedullin conjugated with polyethylene glycol

Keishi Kubo, Mariko Tokashiki, Kenji Kuwasako, Masaji Tamura, more

Peptides > 2014 > 57 > Complete > 118-121

Adrenomedullin (AM) is a vasodilator peptide with pleiotropic effects, including cardiovascular protection and anti-inflammation. Because of these beneficial effects, AM appears to be a promising therapeutic tool for human diseases, while intravenous injection of AM stimulates sympathetic nerve activity due to short-acting potent vasodilation, resulting in increased heart rate and renin secretion...

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Identification of the recognition sequence and target proteins for DJ-1 protease

Hitomi Mitsugi, Takeshi Niki, Kazuko Takahashi-Niki, Kyoko Tanimura, more

FEBS Letters > 2013 > 587 > 16 > 2493-2499

DJ-1, the product of familial Parkinson’s disease gene and an oncogene, is a cysteine protease which plays a role in anti-oxidative stress reaction. In this study, we identified the recognition sequence for DJ-1 protease by using recombinant DJ-1 and a peptide library. Protease activity of DJ-1 lacking C-terminal α-helix (DJ-1ΔH9) was stronger than that of full-sized DJ-1, and the most susceptible...

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Identification of the endogenous cysteine-rich peptide trissin, a ligand for an orphan G protein-coupled receptor in Drosophila

Takanori Ida, Tomoko Takahashi, Hatsumi Tominaga, Takahiro Sato, more

Biochemical and Biophysical Research Communications > 2011 > 414 > 1 > 44-48

There are many orphan G protein-coupled receptors (GPCRs), for which ligands have not yet been identified, in both vertebrates and invertebrates, such as Drosophila melanogaster. Identification of their cognate ligands is critical for understanding the function and regulation of such GPCRs. Indeed, the discovery of bioactive peptides that bind GPCRs has enhanced our understanding of mechanisms underlying...

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Distribution of neuroendocrine regulatory peptide‐1 and ‐2, and proteolytic processing of their precursor VGF protein in the rat

Emi Mishiro‐Sato, Kazuki Sasaki, Takashi Matsuo, Haruaki Kageyama, more

Journal of Neurochemistry > 114 > 4 > 1097 - 1106

J. Neurochem. (2010) 114, 1097–1106. AbstractNeuroendocrine regulatory peptide (NERP)‐1 and NERP‐2 are biologically active peptides recently discovered by peptidomic analysis. NERPs are processed out from the 594‐residue VGF protein which contains many prohormone convertase cleavage motifs. VGF‐deficient mice exhibit a hypermetabolic and infertile phenotype, for which VGF protein‐derived peptides...

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Structural and Functional Study of an Anemonia Elastase Inhibitor, a “Nonclassical” Kazal-Type Inhibitor from Anemonia sulcata ^‡

Hikaru Hemmi, Takashi Kumazaki, Kumiko Yoshizawa-Kumagaye, Yuji Nishiuchi, more

Biochemistry > 2005 > 44 > 28 > 9626 - 9636

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Stability of the O-octanoyl group of rat ghrelin during chemical synthesis: Counter-ion-dependent alteration of an ester bond breakage

Masanori Ishimaru, Kumiko Yoshizawa-Kumagaye, Shigeru Kubo, Tetsuya Kitani, more

International Journal of Peptide Research and Therapeutics > 2003 > 10 > 1 > 41-50

Rat ghrelin, a 28-amino acid residue peptide with an octanoyl group at the side chain of Ser3, was synthesized chemically by applying Fmoc/^tBu strategy. An ester linkage between octanoic acid and the hydroxyl function of Ser3 was found to be maintained without serious damage during the final deprotection with trifluoroacetic acid (TFA). The most notable finding was the counter-ion-dependent stability...

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Site-Dependent Effect of O-Glycosylation on the Conformation and Biological Activity of Calcitonin ^†

Mizuka Tagashira, Hideki Iijima, Yukihiro Isogai, Masayuki Hori, more

Biochemistry > 2001 > 40 > 37 > 11090 - 11095

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Problems associated with use of the N ⁱ ⁿ -cyclohexyloxycarbonyl (Hoc) group for tryptophans

Hideki Nishio, Yuji Nishiuchi, Tatsuya Inui, Kumiko Yoshizawa-Kumagaye, more

Tetrahedron Letters > 2000 > 41 > 35 > 6839-6842

During HF reaction in the presence of anisole or p-cresol, a side reaction was observed associated with use of the N ⁱ ⁿ -cyclohexyloxycarbonyl (Hoc) group for tryptophan (Trp). The structure of the modified Trp and the HF conditions for suppressing this side reaction are described.

article

Synthetic study of phosphopeptides related to heat shock protein HSP27

Tateaki Wakamiya, Ryusaku Togashi, Takatoshi Nishida, Kunio Saruta, more

Bioorganic & Medicinal Chemistry > 1997 > 5 > 1 > 135-145

Two kinds of phosphoserine-containing peptides related to HSP27 were synthesized by the Boc- or Fmoc-mode solid-phase method based on prephosphorylation strategy. In the case of the Boc strategy, the O-phosphono group of the phosphoserine residue was protected with the cyclopentyl or cyclohexyl group. On the other hand, N′-Fmoc-O-[(benzyloxy)hydroxyphosphinyl]serine was employed in case of the Fmoc...

article

Vasopressor Activities of N-Terminal Fragments of Adrenomedullin in Anesthetized Rat

Takushi X. Watanabe, Yukako Itahara, Tatsyuya Inui, Kumiko Yoshizawa-Kumagaye, more

Biochemical and Biophysical Research Communications > 1996 > 219 > 1 > 59-63

Adrenomedullin (AM) is a vasorelaxant peptide that was recently isolated from human pheochromocytoma. In contrast to human (h) AM, which has vasodepressor activity, a synthetic N-terminal fragment of hAM, hAM-(1-25)-NH ₂ showed vasopressor activity in the anesthetized rat. The N-terminal peptides hAM-(1-31)-NH ₂ , hAM-(1-25)-OH, hAM-(1-21)-NH ₂ , acetyl-hAM-(16-21)-NH ₂...

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