Search results for: Anna U. Bielinska

Items from 1 to 13 out of 13 results

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Immunomodulation of TH2 biased immunity with mucosal administration of nanoemulsion adjuvant

Anna U. Bielinska, Jessica J. O’Konek, Katarzyna W. Janczak, James R. Baker

Vaccine > 2016 > 34 > 34 > 4017-4024

T_H2-biased immune responses are associated with inadequate protection against some pathogens and with cancer, colitis, asthma and allergy. Since most currently used vaccine adjuvants induce a T_H2-biased response, this has led to interest in developing adjuvants capable of activating T_H1 immunity and modulating existing T_H2 responses. Immunotherapies to shift immune responses from T_H2 to T_H1 have generally...

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Quantification of cytosolic plasmid DNA degradation using high‐throughput sequencing: implications for gene delivery

Rahul Rattan, Anna U. Bielinska, Mark M. Banaszak Holl

The Journal of Gene Medicine > 16 > 3-4 > 75 - 83

BackgroundAlthough cytosolic DNA degradation plays an important role in decreasing transgene expression, the plasmid degradation pattern remains largely unexplored. MethodsIllumina dye sequencing was employed to provide degradation site information for S1 and cytosolic nucleases. S1 nuclease provided a positive control for a comparison between the agarose gel method and sequencing approaches. ResultsThe...

article

Nanoemulsion nasal adjuvant W805EC induces dendritic cell engulfment of antigen-primed epithelial cells

Andrzej Myc, Jolanta F. Kukowska-Latallo, Douglas M. Smith, Crystal Passmore, more

Vaccine > 2013 > 31 > 7 > 1072-1079

Nanoemulsions are adjuvants that enhance antigen penetration in the nasal mucosa, increase cellular uptake of antigens by both epithelial dendritic cells, and promote migration of antigen-loaded dendritic cells to regional lymph nodes within a day of vaccine administration. The objective of this study was to determine whether the W₈₀5EC nanoemulsion adjuvant enhances immune response not only by direct...

article

Nanoemulsion mucosal adjuvant uniquely activates cytokine production by nasal ciliated epithelium and induces dendritic cell trafficking

Paul E. Makidon, Igor M. Belyakov, Luz P. Blanco, Katarzyna W. Janczak, more

European Journal of Immunology > 42 > 8 > 2073 - 2086

While the nasal mucosa is a potentially useful site for human immunization, toxin‐based nasal adjuvants are generally unsafe and less effective in humans. Safe mucosal adjuvants that activate protective immunity via mucosal administration are highly dependent on barrier antigen sampling by epithelial and DCs. Here, we demonstrate that protein antigens formulated in unique oil‐in‐water nanoemulsions...

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The Role of Ganglioside GM ₁ in Cellular Internalization Mechanisms of Poly(amidoamine) Dendrimers

Seungpyo Hong, Rahul Rattan, István J. Majoros, Douglas G. Mullen, more

Bioconjugate Chemistry > 2009 > 20 > 8 > 1503 - 1513

article

Silver/Dendrimer Nanocomposites as Biomarkers: Fabrication, Characterization, in Vitro Toxicity, and Intracellular Detection

Wojciech Lesniak, Anna U. Bielinska, Kai Sun, Katarzyna W. Janczak, more

Nano Letters > 2005 > 5 > 11 > 2123 - 2130

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Interaction of Poly(amidoamine) Dendrimers with Supported Lipid Bilayers and Cells: Hole Formation and the Relation to Transport

Seungpyo Hong, Anna U. Bielinska, Almut Mecke, Balazs Keszler, more

Bioconjugate Chemistry > 2004 > 15 > 4 > 774 - 782

article

Development of immune response that protects mice from viral pneumonitis after a single intranasal immunization with influenza A virus and nanoemulsion

Andrzej Myc, Jolanta F. Kukowska-Latallo, Anna U. Bielinska, Peter Cao, more

Vaccine > 2003 > 21 > 25-26 > 3801-3814

Nanoemulsion, a water-in-oil formulation stabilized by small amounts of surfactant, is non-toxic to mucous membranes and produces biocidal activity against enveloped viruses. We evaluated nanoemulsion as an adjuvant for mucosal influenza vaccines. Mice (C3H/HeNHsd strain) were vaccinated intranasally with 5x10 ⁵ plaque forming units (pfu) of influenza A virus (Ann Arbor/6/60 strain) and a...

article

Topical transfection using plasmid DNA in a water-in-oil nanoemulsion

Huailiang Wu, Chandrasekharan Ramachandran, Anna U. Bielinska, Kristen Kingzett, more

International Journal of Pharmaceutics > 2001 > 221 > 1-2 > 23 - 34

Expression plasmids encoding chloramphenicol acetyltransferase (CAT) or human interferon-α2 cDNA were formulated in water-in-oil nanoemulsions and applied to murine skin. The histological location of transfected cells was assessed by in situ DNA PCR and showed that the deposition of plasmid DNA was primarily in follicular keratinocytes. Transgene expression in the skin was monitored for 24-72 h, following...

article

Substituted β-Cyclodextrins Interact with PAMAM Dendrimer–DNA Complexes and Modify Transfection Efficiency

Blake J. Roessler, Anna U. Bielinska, Katarzyna Janczak, Inhan Lee, more

Biochemical and Biophysical Research Communications > 2001 > 283 > 1 > 124-129

The efficiency of PAMAM dendrimer-mediated DNA transfer can be improved by the addition of substituted β-cyclodextrins (β-CDs) as formulation excipients. In vitro CAT expression increased approximately 200-fold when dendrimer/DNA/β-CD formulations were applied on the surface of collagen membranes. The inclusion of β-CD into the formulations resulted in particles that were smaller and more evenly distributed...

article

DNA/Dendrimer Complexes Mediate Gene Transfer into Murine Cardiac Transplants ex Vivo

Yinong Wang, Peter Boros, Jianhua Liu, Lihui Qin, more

Molecular Therapy > 2000 > 2 > 6 > 602-608

Starburst polyamidoamine dendrimers are synthetic polymers with unique structural and physical characteristics suitable for DNA gene transfer. Our previous studies demonstrated that Starburst dendrimers augment plasmid-mediated gene transfer efficiency in a nonvascularized, cardiac transplantation model. In this study, the fifth generation of ethylenediamine core dendrimer was investigated for its...

article

The use of PAMAM dendrimers in the efficient transfer of genetic material into cells

Jonathan D. Eichman, Anna U. Bielinska, Jolanta F. Kukowska-Latallo, James R. Baker

Pharmaceutical Science & Technology Today > 2000 > 3 > 7 > 232-245

Polyamidoamine (PAMAM) dendrimers have steadily grown in popularity in the past decade in a variety of disciplines, ranging from materials science to biomedicine. This can be attributed in part to their use in applications that range from computer toners to medical diagnostics. PAMAM dendrimers are safe and nonimmunogenic, and can function as highly efficient cationic polymer vectors for delivering...

article

Enhancement of Dendrimer-Mediated Transfection Using Synthetic Lung Surfactant Exosurf Neonatal in Vitro

Jolanta F. Kukowska-Latallo, Chunling Chen, Jon Eichman, Anna U. Bielinska, more

Biochemical and Biophysical Research Communications > 1999 > 264 > 1 > 253-261

Pulmonary surfactants enhance adenovirus-mediated gene transfer but inhibit cationic liposome-mediated transfection in lung epithelial cells in vitro. This study examines the effect of the synthetic lung surfactant Exosurf on dendrimer-mediated transfection in eukaryotic cells. Exosurf significantly enhanced dendrimer-luciferase plasmid transfection in a number of cell lines and was very effective...

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