Search results for: Andreas Ebner

Items from 1 to 20 out of 61 results

article

Molecular AFM imaging of Hsp70-1A association with dipalmitoyl phosphatidylserine reveals membrane blebbing in the presence of cholesterol

Constanze Lamprecht, Mathias Gehrmann, Josef Madl, Winfried Römer, more

Cell Stress and Chaperones > 2018 > 23 > 4 > 673-683

Hsp70-1A—the major stress-inducible member of the HSP70 chaperone family—is being implicated in cancer diseases with the development of resistances to standard therapies. In normal cells, the protein is purely cytosolic, but in a growing number of tumor cells, a significant fraction can be identified on to the cell surface. The anchoring mechanism is still under debate, as Hsp70-1A lacks conventional...

article

Characterization of the specific interaction between the DNA aptamer sgc8c and protein tyrosine kinase-7 receptors at the surface of T-cells by biosensing AFM

Michael Leitner, Alexandra Poturnayova, Constanze Lamprecht, Sabine Weich, more

Analytical and Bioanalytical Chemistry > 2017 > 409 > 11 > 2767-2776

We studied the interaction of the specific DNA aptamer sgc8c immobilized at the AFM tip with its corresponding receptor, the protein tyrosine kinase-7 (PTK7) embedded in the membrane of acute lymphoblastic leukemia (ALL) cells (Jurkat T-cells). Performing single molecule force spectroscopy (SMFS) experiments, we showed that the aptamer sgc8c bound with high probability (38.3 ± 7.48%) and high specificity...

article

Photopicking: In Situ Approach for Site‐Specific Attachment of Single Multiprotein Nanoparticles to Atomic Force Microscopy Tips

Ivan Liashkovich, Gonzalo Rosso, Martina Rangl, Andreas Ebner, more

Advanced Functional Materials > 27 > 8 > n/a - n/a

Ligand–receptor interactions are fundamental in life sciences and include hormone–receptor, protein–protein, pathogen–host, and cell–cell interactions, among others. Atomic force microscopy (AFM) proved to be invaluable for scrutinizing ligand–receptor interactions at the single molecular level. Basically, a ligand is attached to the AFM tip while its cognate receptor is immobilized on a surface or...

article

Advanced portrayal of SMIL coating by allying CZE performance with in-capillary topographic and charge-related surface characterization

Lorenz G. Stock, Michael Leitner, Lukas Traxler, Klaus Bonazza, more

Analytica Chimica Acta > 2017 > 951 > C > 1-15

A successive multiple ionic polymer layer (SMIL) coating composed of four layers improved the capillary electrophoretic separation of a recombinant major birch pollen allergen and closely related variants when poly(acrylamide-co-2-acrylamido-2-methyl-1-propansulfonate) (55% PAMAMPS) replaced dextran sulfate as terminal SMIL layer. 55% PAMAMPS decelerated the electroosmotic flow (EOF) due to its lower...

article

Mapping molecular adhesion sites inside SMIL coated capillaries using atomic force microscopy recognition imaging

Michael Leitner, Lorenz G. Stock, Lukas Traxler, Laurent Leclercq, more

Analytica Chimica Acta > 2016 > 930 > C > 39-48

Capillary zone electrophoresis (CZE) is a powerful analytical technique for fast and efficient separation of different analytes ranging from small inorganic ions to large proteins. However electrophoretic resolution significantly depends on the coating of the inner capillary surface. High technical efforts like Successive Multiple Ionic Polymer Layer (SMIL) generation have been taken to develop stable...

article

Regenerative biosensor chips based on switchable mutants of avidin—A systematic study

Dominik Zauner, Barbara Taskinen, Daniel Eichinger, Clemens Flattinger, more

Sensors and Actuators B: Chemical > 2016 > 229 > C > 646-654

Biotinylated bait molecules can be immobilized on biotinylated sensor chips by formation of biotin–avidin–biotin bridges which are very stable when using wild-type (strept)avidin. Stable immobilization of biotinylated baits is important for monitoring reversible binding and dissociation of prey molecules. For measurements with another bait molecule, however, it is desirable to replace all immobilized...

chapter

Functionalization of Probe Tips and Supports for Single-Molecule Recognition Force Microscopy

Andreas Ebner, Linda Wildling, Rong Zhu, Christian Rankl, more

Topics in Current Chemistry > STM and AFM Studies on (Bio)molecular Systems: Unravelling the Nanoworld > 29-76

The measuring tip of a force microscope can be converted into a monomolecular sensorif one or few “ligand” molecules are attached to the apex of the tip while maintainingligand function. Functionalized tips are used to study fine details of receptor–ligand interactionby force spectroscopy or to map cognate “receptor” molecules on the sample surface. Thereceptor (or target) molecules can be present...

chapter

Molecular Recognition Force Microscopy: From Molecular Bonds to Complex Energy Landscapes

Peter Hinterdorfer, Andreas Ebner, Hermann Gruber, Ruti Kapon, more

Nanotribology and Nanomechanics I > Scanning Probe Microscopy > 355-387

Atomic force microscopy (AFM), atomic force microscopy (AFM) developed in the late 1980s to explore atomic details on hard material surfaces, has evolved into a method capable of imaging fine structural details of biological samples. Its particular advantage in biology is that measurements can be carried out in aqueous and physiological environments, which opens the possibility to study the dynamics...

chapter

Recognition Imaging Using Atomic Force Microscopy

Andreas Ebner, Lilia Chtcheglova, Jilin Tang, David Alsteens, more

Handbook of Single-Molecule Biophysics > 525-554

Recognition imaging using atomic force microscopy (AFM) offers a wealth of new opportunities in biophysical research, such as its ability to localize specific chemical groups and biological receptors on biosurfaces and to measure their molecular-scale interactions. By attaching well-defined chemical groups on tips, it is possible to map chemical properties and interactions on cell surfaces on a scale...

chapter

Probing the Energy Landscape of Protein-Binding Reactions by Dynamic Force Spectroscopy

Andreas Ebner, Reinat Nevo, Christian Ranki, Johannes Preiner, more

Handbook of Single-Molecule Biophysics > 407-447

This chapter describes how the energy landscape that underlies protein-binding reactions can be revealed using dynamic force spectroscopy. The chapter begins with a detailed description of methodologies used and requirements of the experimental system, including tip and surface materials and their functionalization strategies. The next few sections discuss the fundamentals of measuring forces using...

chapter

Molecular Recognition Force Microscopy: From Molecular Bonds to Complex Energy Landscapes

Peter Hinterdorfer, Andreas Ebner, Hermann Gruber, Ruti Kapon, more

Nanotribology and Nanomechanics I > Introduction – Measurement Techniques and Applications > 355-387

chapter

Exploring Carbon Nanotubes and Their Interaction with Cells Using Atomic Force Microscopy

Constanze Lamprecht, Andreas Ebner, Ferry Kienberger, Peter Hinterdorfer

Carbon Nanostructures > Carbon Nanotubes for Biomedical Applications > Interaction with biological systems > 1-16

At present, atomic force microscopy (AFM) offers a unique solution to study biological specimens on the nanometer scale under near-physiological conditions without the need for rigorous sample preparation, staining or labelling. We expect new and significant biophysical insights into the delivery process and transport mechanism of CNTs into cells employing AFM. Here we give an overview for the application...

chapter

Nanoimaging, Molecular Interaction, and Nanotemplating of Human Rhinovirus

Markus Kastner, Christian Rankl, Andreas Ebner, Philipp D. Pollheimer, more

NanoScience and Technology > Scanning Probe Microscopy in Nanoscience and Nanotechnology 2 > Industrial Applications > 589-643

Capturing and detecting viruses require specialized platforms and analysis techniques. The atomic force microscope (AFM) offers not only tremendously high spatial and topographic resolution, but also allows localizing single molecules and determining their interaction on a molecular level. AFM makes possible (a) generating nanopatterned surfaces, (b) detecting protein and viral pathogen captured in...

chapter

Molecular Recognition Force Microscopy: From Molecular Bonds to Complex Energy Landscapes

Peter Hinterdorfer, Andreas Ebner, Hermann Gruber, Ruti Kapon, more

Springer Handbook of Nanotechnology > Scanning-Probe Microscopy > 763-785

article

Aptamer-based detection of thrombin by acoustic method using DNA tetrahedrons as immobilisation platform

Alexandra Poturnayová, Maja Šnejdárková, Gabriela Castillo, Peter Rybár, more

Chemical Papers > 2015 > 69 > 1 > 211-226

The thickness shear mode acoustic method was used to study the binding of thrombin to DNA aptamers immobilised on the gold surface covered by DNA tetrahedrons. The binding of thrombin to conventional aptamers sensitive to fibrinogen (FBT) and heparin (HPT) exosites as well as to HPT in a loop configuration (HPTloop) made it possible to determine the constant of dissociation (K_D) and the limit of detection...

article

Novel Generation of Crosslinkers allows Single Molecule Force Spectroscopy on Oligomeric Receptors

Doris Sinwel, Andreas Karner, Andreas Ebner, Rong Zhu, more

Biophysical Journal > 2014 > 106 > 2 Supplement 1 > 387a

article

Investigating the binding behaviour of two avidin‐based testosterone binders using molecular recognition force spectroscopy

Martina Rangl, Michael Leitner, Tiina Riihimäki, Soili Lehtonen, more

Journal of Molecular Recognition > 27 > 2 > 92 - 97

Molecular recognition force spectroscopy, a biosensing atomic force microscopy technique allows to characterise the dissociation of ligand–receptor complexes at the molecular level. Here, we used molecular recognition force spectroscopy to study the binding capability of recently developed testosterone binders. The two avidin‐based proteins called sbAvd‐1 and sbAvd‐2 are expected to bind both testosterone...

article

Kinetics of bioconjugate nanoparticle label binding in a sandwich-type immunoassay

Tuomas Näreoja, Andreas Ebner, Hermann J. Gruber, Barbara Taskinen, more

Analytical and Bioanalytical Chemistry > 2014 > 406 > 2 > 493-503

Nanoparticle labels have enhanced the performance of diagnostic, screening, and other measurement applications and hold further promise for more sensitive, precise, and cost-effective assay technologies. Nevertheless, a clear view of the biomolecular interactions on the molecular level is missing. Controlling the ratio of molecular recognition over undesired nonspecific adhesion is the key to improve...

article

Single‐Molecule Analysis of the Recognition Forces Underlying Nucleo‐Cytoplasmic Transport

Martina Rangl, Andreas Ebner, Justin Yamada, Christian Rankl, more

Angewandte Chemie > 125 > 39 > 10546 - 10549

Transportkräfte: Damit Moleküle sich durch die Kernhülle bewegen können, müssen sie selektive Barrieren der Kernpore überwinden, die aus Nucleoporinen (nups) mit FG‐Wiederholungen bestehen. Die Moleküle werden von pendelnden Rezeptoren begleitet, die mit den FG‐nups wechselwirken, und passieren unter Verwendung eines ungeklärten Mechanismus die Barriere. Der Prozess wurde mit Einzelmolekülspektroskopie...

article

Single‐Molecule Analysis of the Recognition Forces Underlying Nucleo‐Cytoplasmic Transport

Martina Rangl, Andreas Ebner, Justin Yamada, Christian Rankl, more

Angewandte Chemie International Edition > 52 > 39 > 10356 - 10359

Transport forces: To move molecules across the nuclear envelope they have to overcome the selective barrier of the nuclear pore which is formed by nucleoporins (nups) with FG repeats. The molecules are chaperoned by shuttling receptors that interact with FG nups thereby passing the barrier using an unresolved mechanism. This process is explored by single‐molecule force spectroscopy (see picture).

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ATOMIC FORCE MICROSCOPY (8)
MOLECULAR RECOGNITION (4)
SINGLE MOLECULE FORCE SPECTROSCOPY (3)
AFM (2)
AVIDIN (2)
DNA APTAMER (2)
FORCE SPECTROSCOPY (2)
MEMBRANES (2)
MOLEKULARE ERKENNUNG (2)
RECOGNITION (2)
RECOGNITION IMAGING (2)
SILICON NITRIDE (2)
SMIL COATING (2)
THROMBIN (2)
ETHER-À-GO-GO (1)
ADHESION FORCES (1)
AMINOSILANE (1)
ANTIBODY (1)
APTAMERS (1)
APTASENSOR (1)
ATOMIC FORCE MICROSCOPE (1)
ATOMIC FORCE SPECTROSCOPY (1)
AVIDIN MUTANT (1)
AVIDIN/BIOTIN INTERACTION (1)
AVIDIN–BIOTIN (1)
BIOCONJUGATION (1)
BIOFUNCTIONALIZED NANOPARTICLE LABELS (1)
BIOLAYER INTERFEROMETRY (1)
BIONANOPARTICLES (1)
BIOSENSOR (1)
BIOTIN (1)
BIOTIN SURFACE (1)
BONE MORPHOGENETIC PROTEIN-2 (BMP-2) (1)
BONE REGENERATION (1)
BRANCHING MORPHOGENESIS (1)
CANCER (1)
CAPILLARY ELECTROPHORESIS (1)
CAPILLARY ZONE ELECTROPHORESIS (1)
CELL MIGRATION (1)
CHLOROQUINE (1)
CINCHONA ALKALOIDS (1)
CINCHONA‐ALKALOIDE (1)
CY3B (1)
CYDYE (1)
DNA CONDENSATION (1)
DNA STRUCTURES (1)
DNA TETRAHEDRONS (1)
DNA‐STRUKTUREN (1)
DPPS (1)
ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY (1)
EMPTY SPIRACLES (1)
ENERGY LANDSCAPE (1)
ETHANOLAMINE (1)
FAST AFM IMAGING (1)
FLUORESCENCE (1)
FLÜSSIGCHROMATOGRAPHIE (1)
FORCE MICROSCOPY (1)
FORCE–VOLUME (1)
FUNCTIONALIZATION (1)
HEAT SHOCK PROTEIN (1)
HETEROBIFUNCTIONAL CROSSLINKER (1)
HIGH RESOLUTION IMAGING (1)
HIS-TAG (1)
HSP70 (1)
HUMAN RHINOVIRUS (1)
IMAGING (1)
INTEGRIN ΑIIBΒ3 (1)
INTERCALATION (1)
K CHANNEL (1)
KERNPOREN (1)
KRAFTSPEKTROSKOPIE (1)
LIQUID CHROMATOGRAPHY (1)
MESENCHYMAL STEM CELLS (1)
MOLECULAR RECOGNITION FORCE SPECTROSCOPY (1)
NANOCRYSTALLINE DIAMOND (NCD) (1)
NANOMEDICINE (1)
NANORODS (1)
NANOTECHNOLOGY (1)
NANOTOPOGRAPHY (1)
NONSPECIFIC BINDING (1)
NUCLEAR PORE COMPLEXES (1)
NUCLEOPORINE (1)
NUCLEOPORINS (1)
PHASE (1)
PHOTO‐CROSS‐LINKING (1)
PHYSISORPTION (1)
PLASMID DNA (1)
PLASMID TOPOISOMERS (1)
PLASMIDTOPOISOMERE (1)
PLATELETS (1)
POLY(ETHYLENE GLYCOL) (1)
PROTEIN RECOGNITION (1)
PROTEIN SEPARATION (1)
PROTEOGLYCAN (1)
PSEUDOEXFOLIATION SYNDROME (1)
PTK7 (1)
RASTERKRAFTMIKROSKOPIE (1)
RECOGNITION FORCE SPECTROSCOPY (1)
REVERSIBLE IMMOBILIZATION (1)
S-LAYER PROTEIN (1)
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Bioconjugate Chemistry (4)
Analytical and Bioanalytical Chemistry (3)
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Angewandte Chemie (2)
Angewandte Chemie International Edition (2)
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Advanced Functional Materials (1)
Applied Physics A (1)
Biomaterials (1)
Cell Stress and Chaperones (1)
Chemical Papers (1)
Chemical Physics Letters (1)
Developmental Cell (1)
Electroanalysis (1)
Gene (1)
Journal of Fluorescence (1)
Journal of Molecular Recognition (1)
Journal of Structural Biology (1)
Langmuir (1)
Methods (1)
Micron (1)
Nano Letters (1)
Pflügers Archiv - European Journal of Physiology (1)
Sensors and Actuators B: Chemical (1)
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INFONA - science communication portal

Search results for: Andreas Ebner

Molecular AFM imaging of Hsp70-1A association with dipalmitoyl phosphatidylserine reveals membrane blebbing in the presence of cholesterol

Characterization of the specific interaction between the DNA aptamer sgc8c and protein tyrosine kinase-7 receptors at the surface of T-cells by biosensing AFM

Photopicking: In Situ Approach for Site‐Specific Attachment of Single Multiprotein Nanoparticles to Atomic Force Microscopy Tips

Advanced portrayal of SMIL coating by allying CZE performance with in-capillary topographic and charge-related surface characterization

Mapping molecular adhesion sites inside SMIL coated capillaries using atomic force microscopy recognition imaging

Regenerative biosensor chips based on switchable mutants of avidin—A systematic study

Functionalization of Probe Tips and Supports for Single-Molecule Recognition Force Microscopy

Molecular Recognition Force Microscopy: From Molecular Bonds to Complex Energy Landscapes

Recognition Imaging Using Atomic Force Microscopy

Probing the Energy Landscape of Protein-Binding Reactions by Dynamic Force Spectroscopy

Molecular Recognition Force Microscopy: From Molecular Bonds to Complex Energy Landscapes

Exploring Carbon Nanotubes and Their Interaction with Cells Using Atomic Force Microscopy

Nanoimaging, Molecular Interaction, and Nanotemplating of Human Rhinovirus

Molecular Recognition Force Microscopy: From Molecular Bonds to Complex Energy Landscapes

Aptamer-based detection of thrombin by acoustic method using DNA tetrahedrons as immobilisation platform

Novel Generation of Crosslinkers allows Single Molecule Force Spectroscopy on Oligomeric Receptors

Investigating the binding behaviour of two avidin‐based testosterone binders using molecular recognition force spectroscopy

Kinetics of bioconjugate nanoparticle label binding in a sandwich-type immunoassay

Single‐Molecule Analysis of the Recognition Forces Underlying Nucleo‐Cytoplasmic Transport

Single‐Molecule Analysis of the Recognition Forces Underlying Nucleo‐Cytoplasmic Transport

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