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A method for fluorescent sensing of thrombin was developed by using fluorescent silica nanoparticles as the fluorescence signal probes and magnetic beads as target enriching platform. RuBpy-doped silica nanoparticles are highly photostable and provide significant fluorescent signal amplification as compared with single dye molecules. TBA1 is a 15-mer DNA aptamer which binds exosite I of thrombin (Fibrinogen...
We present a simple and sensitive method for multiplexed DNA detection by simultaneously capturing two different DNA sequences with a same silica nanoparticle (NP) through a sandwich mode. This biobarcode assay method was demonstrated by using oligonucleotide sequences of 64 bases associated with human papillomavirus (HPV) 16 and 18 L1 genes as model systems. The nonfluorescent carboxyl-modified silica...
In this work, we have developed a simple and sensitive method for ATP detection using silica nanoparticles (NPs) as the platform and hoechst33258 as the signal reporter. The ATP-binding aptamers hybridize with the probe DNA (DNAp) immobilized NPs to form the aptamer/DNAp duplex on the NPs surface. The conformational change of the aptamer leads to the decrease of the aptamer/DNAp duplex on the NPs...
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