Search results for: Jung-Hye Roe

Items from 1 to 20 out of 33 results

article

Activity modulation of anti‐sigma factor via cysteine alkylation in Actinobacteria

Gyeong‐Seok Oh, Ji‐Sun Yoo, Joo‐Hong Park, Jung‐Hye Roe

Molecular Microbiology > 117 > 2 > 539 - 550

σ^R (SigR) is an alternative sigma factor that enables gene expression in Streptomyces coelicolor to cope with thiol oxidation and antibiotic stresses. Its activity is repressed by a zinc‐containing anti‐sigma (ZAS) factor RsrA that senses thiol oxidants and electrophiles. Inactivation of RsrA by disulfide formation has been well studied. Here we investigated another pathway of RsrA inactivation by...

article

uORF‐mediated riboregulation controls transcription of whiB7/wblC antibiotic resistance gene

Ju‐Hyung Lee, Eun‐Jin Lee, Jung‐Hye Roe

Molecular Microbiology > 117 > 1 > 179 - 192

WhiB7/WblC is a transcriptional factor of actinomycetes conferring intrinsic resistance to multiple translation‐inhibitory antibiotics. It positively autoregulates its own transcription in response to the same antibiotics. The presence of a uORF and a potential Rho‐independent transcription terminator in the 5′ leader region has suggested a possibility that the whiB7/wblC gene is regulated via a uORF‐mediated...

article

The role of Rsv1 in the transcriptional regulation of genes involved in sugar metabolism for long‐term survival

Eun‐Jung Kim, Yong‐Joon Cho, Woo‐Hyun Chung, Jung‐Hye Roe

The FEBS Journal > 287 > 5 > 878 - 896

Glucose limitation is a major stress condition that cells must respond to by altering their metabolism to ensure survival. Rsv1 is a zinc finger protein previously shown to be required for survival during stationary phase. In this study, we present a novel mechanism regulated by Rsv1 in the fission yeast Schizosaccharomyces pombe that is involved in altering glucose metabolic flux. We found that rsv1...

article

SigR, a hub of multilayered regulation of redox and antibiotic stress responses

Joo‐Hong Park, Ju‐Hyung Lee, Jung‐Hye Roe

Molecular Microbiology > 112 > 2 > 420 - 431

Signal‐specific activation of alternative sigma factors redirects RNA polymerase to induce transcription of distinct sets of genes conferring protection against the damage the signal and the related stresses incur. In Streptomyces coelicolor, σ^R (SigR), a member of ECF12 subfamily of Group IV sigma factors, responds to thiol‐perturbing signals such as oxidants and electrophiles, as well as to translation‐blocking...

article

Improved production of clavulanic acid by reverse engineering and overexpression of the regulatory genes in an industrial Streptomyces clavuligerus strain

Hang Soo Cho, Jin Chul Jo, Chang-Hun Shin, Namil Lee, more

Journal of Industrial Microbiology & Biotechnology > 2019 > 46 > 8 > 1205-1215

Genomic analysis of the clavulanic acid (CA)-high-producing Streptomyces clavuligerus strains, OL13 and OR, developed through random mutagenesis revealed a frameshift mutation in the cas1 gene-encoding clavaminate synthase 1. Overexpression of the intact cas1 in S. clavuligerus OR enhanced the CA titer by approximately 25%, producing ~ 4.95 g/L of CA, over the OR strain in the flask culture. Moreover,...

article

Growth and differentiation properties of pikromycin-producing Streptomyces venezuelae ATCC15439

Ji-Eun Kim, Joon-Sun Choi, Jung-Hye Roe

Journal of Microbiology > 2019 > 57 > 5 > 388-395

Streptomycetes naturally produce a variety of secondary metabolites, in the process of physiological differentiation. Streptomyces venezuelae differentiates into spores in liquid media, serving as a good model system for differentiation and a host for exogenous gene expression. Here, we report the growth and differentiation properties of S. venezuelae ATCC-15439 in liquid medium, which produces pikromycin,...

article

The iron uptake repressor Fep1 in the fission yeast binds Fe-S cluster through conserved cysteines

Hyo-Jin Kim, Kang-Lok Lee, Kyoung-Dong Kim, Jung-Hye Roe

Biochemical and Biophysical Research Communications > 2016 > 478 > 1 > 187-192

Iron homeostasis is tightly regulated since iron is an essential but toxic element in the cell. The GATA-type transcription factor Fep1 and its orthologs contribute to iron homeostasis in many fungi by repressing genes for iron uptake when intracellular iron is high. Even though the function and interaction partners of Fep1 have been elucidated extensively In Schizosaccharomyces pombe, the mechanism...

article

Complete genome sequence of Streptomyces venezuelae ATCC 15439, a promising cell factory for production of secondary metabolites

Ju Yeon Song, Young Ji Yoo, Si-Kyu Lim, Sun Ho Cha, more

Journal of Biotechnology > 2016 > 219 > C > 57-58

Streptomyces venezuelae ATCC 15439, which produces 12- and 14-membered ring macrolide antibiotics, is a platform strain for heterologous expression of secondary metabolites. Its 9.05-Mb genome sequence revealed an abundance of genes involved in the biosynthesis of secondary metabolites and their precursors, which should be useful for the production of bioactive compounds.

article

Factors affecting redox potential and differential sensitivity of SoxR to redox‐active compounds

Kang‐Lok Lee, Atul K. Singh, Lim Heo, Chaok Seok, more

Molecular Microbiology > 97 > 5 > 808 - 821

SoxR is a [2Fe‐2S]‐containing sensor‐regulator, which is activated through oxidation by redox‐active compounds (RACs). SoxRs show differential sensitivity to RACs, partly due to different redox potentials, such that Escherichia coli (Ec) SoxR with lower potential respond to broader range of RACs than Streptomyces coelicolor (Sc) SoxR. In S. coelicolor, the RACs that do not activate ScSoxR did not...

article

Essential function of Aco2, a fusion protein of aconitase and mitochondrial ribosomal protein bL21, in mitochondrial translation in fission yeast

Soo-Jin Jung, Youngdae Seo, Kyung-Chang Lee, Daeyoup Lee, more

FEBS Letters > 2015 > 589 > 7 > 822-828

A possible interaction between aconitase and a mitochondrial ribosomal protein was suggested in a genome-wide interactome study. In fission yeast Schizosaccharomyces pombe, the aco2⁺ gene encodes a fusion protein between aconitase and a putative mitochondrial ribosomal protein bL21 (Mrpl49). Two types of aco2⁺ transcripts are generated via alternative poly (A) site selection, producing both a single...

article

Essential function of Aco2, a fusion protein of aconitase and mitochondrial ribosomal protein bL21, in mitochondrial translation in fission yeast

Soo-Jin Jung, Youngdae Seo, Kyung-Chang Lee, Daeyoup Lee, more

FEBS Letters > 589 > 7 > 822 - 828

A possible interaction between aconitase and a mitochondrial ribosomal protein was suggested in a genome‐wide interactome study. In fission yeast Schizosaccharomyces pombe, the aco2⁺ gene encodes a fusion protein between aconitase and a putative mitochondrial ribosomal protein bL21 (Mrpl49). Two types of aco2 ⁺ transcripts are generated via alternative poly (A) site selection, producing both a single...

article

Genome-scale analysis reveals a role for NdgR in the thiol oxidative stress response in Streptomyces coelicolor

Ji-Nu Kim, Yujin Jeong, Ji Sun Yoo, Jung-Hye Roe, more

BMC Genomics > 2015 > 16 > 1 > 1-11

Background NdgR is an IclR-type transcription factor that regulates leucine biosynthesis and other metabolic pathways in Streptomyces coelicolor . Recent study revealed that NdgR is one of the regulatory targets of SigR, an oxidative stress response sigma factor, suggesting that the NdgR plays an important physiological role in response to environmental stresses. Although the...

article

Comparative study of SoxR activation by redox‐active compounds

Atul K. Singh, Jung‐Ho Shin, Kang‐Lok Lee, James A. Imlay, more

Molecular Microbiology > 90 > 5 > 983 - 996

SoxR from Escherichia coli and related enterobacteria is activated by a broad range of redox‐active compounds through oxidation or nitrosylation of its [2Fe–2S] cluster. Activated SoxR then induces SoxS, which subsequently activates more than 100 genes in response. In contrast, non‐enteric SoxRs directly activate their target genes in response to redox‐active compounds that include endogenously produced...

article

Alternative zinc‐binding sites explain the redox sensitivity of zinc‐containing anti‐sigma factors

Lim Heo, Yoo‐Bok Cho, Myeong Sup Lee, Jung‐Hye Roe, more

Proteins: Structure, Function, and Bioinformatics > 81 > 9 > 1644 - 1652

Certain bacterial zinc‐containing anti‐sigma (ZAS) factors respond sensitively to thiol‐induced oxidative stress by undergoing conformational changes, which in turn reduce binding affinities for their cognate sigma factors. This redox sensitivity provides a mechanism for coping with oxidative stress by activating the transcription of antioxidant genes. Not all ZAS proteins are redox‐sensitive, but...

article

Post-translational regulation of a developmental catalase, CatB, involves a metalloprotease, SmpA and contributes to proper differentiation and osmoprotection of Streptomyces coelicolor

Hyo-Sub Kim, Eun-Jin Lee, You-Hee Cho, Jung-Hye Roe

Research in Microbiology > 2013 > 164 > 4 > 327-334

Streptomyces coelicolor produces at least three different catalases (catalases A, B, and C) under different physiological conditions. Catalase B (CatB) is a developmentally regulated catalase required for proper differentiation and osmoprotection of S. coelicolor. We previously observed that the N-terminal 75 ² 2The 75 aa was once designated as 95 aa in our previous study (Cho et al., 2000),...

article

Conservation of thiol‐oxidative stress responses regulated by SigR orthologues in actinomycetes

Min‐Sik Kim, Yann S. Dufour, Ji Sun Yoo, Yoo‐Bok Cho, more

Molecular Microbiology > 85 > 2 > 326 - 344

Numerous thiol‐reactive compounds cause oxidative stress where cells counteract by activation of survival strategies regulated by thiol‐based sensors. In Streptomyces coelicolor, a model actinomycete, a sigma/antisigma pair SigR/RsrA controls the response to thiol‐oxidative stress. To unravel its full physiological functions, chromatin immuno‐precipitation combined with sequence and transcript analyses...

article

Corrigendum to “Multi-domain CGFS-type glutaredoxin Grx4 regulates iron homeostasis via direct interaction with a repressor Fep1 in fission yeast” [Biochem. Biophys. Res. Commun. 408 (2011) 609–614]

Kyoung-Dong Kim, Hyo-Jin Kim, Kyung-Chang Lee, Jung-Hye Roe

Biochemical and Biophysical Research Communications > 2012 > 418 > 3 > 584

article

Corrigendum to “Monothiol glutaredoxin Grx5 interacts with Fe–S scaffold proteins Isa1 and Isa2 and supports Fe–S assembly and DNA integrity in mitochondria of fission yeast” [Biochem. Biophys. Res. Commun. 392 (2010) 467–472]

Kyoung-Dong Kim, Woo-Hyun Chung, Hyo-Jin Kim, Kyung-Chang Lee, more

Biochemical and Biophysical Research Communications > 2012 > 418 > 3 > 585

article

Multi-domain CGFS-type glutaredoxin Grx4 regulates iron homeostasis via direct interaction with a repressor Fep1 in fission yeast

Kyoung-Dong Kim, Hyo-Jin Kim, Kyung-Chang Lee, Jung-Hye Roe

Biochemical and Biophysical Research Communications > 2011 > 408 > 4 > 609-614

The fission yeast Schizosaccharomyces pombe contains two CGFS-type monothiol glutaredoxins, Grx4 and Grx5, which are localized primarily in the nucleus and mitochondria, respectively. We observed involvement of Grx4 in regulating iron-responsive gene expression, which is modulated by a repressor Fep1. Lack of Grx4 caused defects not only in growth but also in the expression of both iron-uptake and...

article

Capillary Based Patterning of Cellular Communities in Laterally Open Channels

Sung Hoon Lee, Austen James Heinz, Sunghwan Shin, Yong-Gyun Jung, more

Analytical Chemistry > 2010 > 82 > 7 > 2900 - 2906

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INFONA - science communication portal

Search results for: Jung-Hye Roe

Activity modulation of anti‐sigma factor via cysteine alkylation in Actinobacteria

uORF‐mediated riboregulation controls transcription of whiB7/wblC antibiotic resistance gene

The role of Rsv1 in the transcriptional regulation of genes involved in sugar metabolism for long‐term survival

SigR, a hub of multilayered regulation of redox and antibiotic stress responses

Improved production of clavulanic acid by reverse engineering and overexpression of the regulatory genes in an industrial Streptomyces clavuligerus strain

Growth and differentiation properties of pikromycin-producing Streptomyces venezuelae ATCC15439

The iron uptake repressor Fep1 in the fission yeast binds Fe-S cluster through conserved cysteines

Complete genome sequence of Streptomyces venezuelae ATCC 15439, a promising cell factory for production of secondary metabolites

Factors affecting redox potential and differential sensitivity of SoxR to redox‐active compounds

Essential function of Aco2, a fusion protein of aconitase and mitochondrial ribosomal protein bL21, in mitochondrial translation in fission yeast

Essential function of Aco2, a fusion protein of aconitase and mitochondrial ribosomal protein bL21, in mitochondrial translation in fission yeast

Genome-scale analysis reveals a role for NdgR in the thiol oxidative stress response in Streptomyces coelicolor

Comparative study of SoxR activation by redox‐active compounds

Alternative zinc‐binding sites explain the redox sensitivity of zinc‐containing anti‐sigma factors

Post-translational regulation of a developmental catalase, CatB, involves a metalloprotease, SmpA and contributes to proper differentiation and osmoprotection of Streptomyces coelicolor

Conservation of thiol‐oxidative stress responses regulated by SigR orthologues in actinomycetes

Corrigendum to “Multi-domain CGFS-type glutaredoxin Grx4 regulates iron homeostasis via direct interaction with a repressor Fep1 in fission yeast” [Biochem. Biophys. Res. Commun. 408 (2011) 609–614]

Corrigendum to “Monothiol glutaredoxin Grx5 interacts with Fe–S scaffold proteins Isa1 and Isa2 and supports Fe–S assembly and DNA integrity in mitochondria of fission yeast” [Biochem. Biophys. Res. Commun. 392 (2010) 467–472]

Multi-domain CGFS-type glutaredoxin Grx4 regulates iron homeostasis via direct interaction with a repressor Fep1 in fission yeast

Capillary Based Patterning of Cellular Communities in Laterally Open Channels

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