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We invented an electrocatalytic chronopotentiometric stripping (CPS) method for the analysis of virtually all proteins at femtomole level. Sensitivity of this method to changes in protein structures was applied in biomedicine. Polysaccharides and ribose at the 3'-end of RNA can be modified by some Os complexes and detected electrochemically directly in the reaction mixture at nanomolar and subnanomolar...
Monoclonal antibodies (MAbs) were used to investigate the binding of insect cell-expressed, wild-type human p53 protein to the consensus sequence (p53CON) in a 474-bp DNA fragment and to supercoiled (sc) DNAs with and without p53CON. Supershifting of p53-DNA complexes by MAbs in agarose gels was applied to studies of activation of p53 for sequence-specific binding within scDNA. C-terminal specific...
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