Search results for: Leila Birolo

Items from 1 to 20 out of 26 results

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Evolved Gas Analysis‐Mass Spectrometry to Identify the Earliest Organic Binder in Aegean Style Wall Paintings

Ravit Linn, Ilaria Bonaduce, Georgia Ntasi, Leila Birolo, more

Angewandte Chemie > 130 > 40 > 13441 - 13444

An organic binder was identified in the painted fragments from the Canaanite palace of Tel Kabri, Israel. Recently dated to the late 18th century B.C.E. by ¹⁴C, Tel Kabri is the most ancient of the Eastern Mediterranean sites in which Aegean style paintings have been found. The application of pigments was suspected to be using an organic binding medium, particularly for the Egyptian Blue pigment....

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Evolved Gas Analysis‐Mass Spectrometry to Identify the Earliest Organic Binder in Aegean Style Wall Paintings

Ravit Linn, Ilaria Bonaduce, Georgia Ntasi, Leila Birolo, more

Angewandte Chemie International Edition > 57 > 40 > 13257 - 13260

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RHA-P: Isolation, expression and characterization of a bacterial α-l-rhamnosidase from Novosphingobium sp. PP1Y

Federica De Lise, Francesca Mensitieri, Vincenzo Tarallo, Nicola Ventimiglia, more

Journal of Molecular Catalysis B: Enzymatic > 2016 > 134 > PA > 136-147

α-l-Rhamnosidases (α-RHAs) are a group of glycosyl hydrolases of biotechnological potential in industrial processes, which catalyze the hydrolysis of α-l-rhamnose terminal residues from several natural compounds. A novel α–RHA activity was identified in the crude extract of Novosphingobium sp. PP1Y, a marine bacterium able to grow on a wide range of aromatic polycyclic compounds. In this work, this...

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A hypothesis on different technological solutions for outdoor and indoor Roman wall paintings

Leila Birolo, Antonella Tomeo, Marco Trifuoggi, Finizia Auriemma, more

Archaeological and Anthropological Sciences > 2017 > 9 > 4 > 591-602

The determination of the chemical composition of different parts of wall paintings (pigments, mortars and binders) provides information about technology of preparation of an artefact. Herein, we present a multi-methodological characterisation of wall paintings from a Roman archaeological site in Cuma, focusing on differences between an indoor (domus) and outdoor fabrication (a temple, Tempio con Portico...

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Regulating levels of the neuromodulator d‐serine in human brain: structural insight into pLG72 and d‐amino acid oxidase interaction

Leila Birolo, Silvia Sacchi, Giovanni Smaldone, Gianluca Molla, more

The FEBS Journal > 283 > 18 > 3353 - 3370

The human flavoenzyme d‐amino acid oxidase (hDAAO) degrades the NMDA‐receptor modulator d‐serine in the brain. Although hDAAO has been extensively characterized, little is known about its main modulator pLG72, a small protein encoded by the primate‐specific gene G72 that has been associated with schizophrenia susceptibility. pLG72 interacts with neosynthesized hDAAO, promoting its inactivation and...

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Proteomic strategies for cultural heritage: From bones to paintings

Roberto Vinciguerra, Addolorata De Chiaro, Piero Pucci, Gennaro Marino, more

Microchemical Journal > 2016 > 126 > C > 341-348

In recent years, proteomics procedures have become increasingly popular for the characterization of proteinaceous materials in ancient samples of several cultural heritage objects. The knowledge of the materials used in a work of art is crucial, not only to give an insight in the historical context of objects and artists, but also to analyse degradation processes taking place in aged objects and to...

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Femtosecond UV-laser pulses to unveil protein–protein interactions in living cells

Francesco Itri, Daria M. Monti, Bartolomeo Della Ventura, Roberto Vinciguerra, more

Cellular and Molecular Life Sciences > 2016 > 73 > 3 > 637-648

A hallmark to decipher bioprocesses is to characterize protein–protein interactions in living cells. To do this, the development of innovative methodologies, which do not alter proteins and their natural environment, is particularly needed. Here, we report a method (LUCK, Laser UV Cross-linKing) to in vivo cross-link proteins by UV-laser irradiation of living cells. Upon irradiation of HeLa cells...

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Application of a new xylanase activity from Bacillus amyloliquefaciens XR44A in brewer's spent grain saccharification

Antonella Amore, Binod Parameswaran, Ramesh Kumar, Leila Birolo, more

Journal of Chemical Technology & Biotechnology > 90 > 3 > 573 - 581

BACKGROUNDCellulases and xylanases are the key enzymes involved in the conversion of lignocelluloses into fermentable sugars. Western Ghat region (India) has been recognized as an active hot spot for the isolation of new microorganisms. The aim of this work was to isolate new microorganisms producing cellulases and xylanases to be applied in brewer's spent grain saccharification. RESULTS93 microorganisms...

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Reply to Ira Rabin's Comment on our paper Rasmussen et al. (2012)

Kaare Lund Rasmussen, Anna Lluveras Tenorio, Ilaria Bonaduce, Maria Perla Colombini, more

Journal of Archaeological Science > 2014 > 43 > Complete > 155-158

In the Comment by Dr. I. Rabin from Bundesanstalt für Materialforschung und -prüfung in Berlin are stated many conjectures and apparent guesses contradicting several of our interpretations. Most of Rabin's ‘guesses’ and interpretations are unwarranted. Below we rebut some of the misinterpretations under separate headings.

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Ultraviolet laser‐induced cross‐linking in peptides

Gabriella Leo, Carlo Altucci, Sandrine Bourgoin‐Voillard, Alfredo M. Gravagnuolo, more

Rapid Communications in Mass Spectrometry > 27 > 14 > 1660 - 1668

RATIONALEThe aim of this study was to demonstrate, and to characterize by high‐resolution mass spectrometry that it is possible to preferentially induce covalent cross‐links in peptides by using high‐energy femtosecond ultraviolet (UV) laser pulses. The cross‐link is readily formed only when aromatic amino acids are present in the peptide sequence. METHODSThree peptides, xenopsin, angiotensin I, and...

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Industrial waste based compost as a source of novel cellulolytic strains and enzymes

Antonella Amore, Olimpia Pepe, Valeria Ventorino, Leila Birolo, more

FEMS Microbiology Letters > 339 > 2 > 93 - 101

Ninety bacteria isolated from raw composting materials were screened for their cellulolytic activity on solid medium containing carboxymethylcellulose. The bacteria producing the highest cellulolytic activity levels were identified by 16S rRNA sequencing as Bacillus licheniformis strain 1, Bacillus subtilis subsp. subtilis strain B7B,Bacillus subtilis subsp. spizizenii strain 6, and Bacillus amyloliquefaciens...

article

A simple and reliable methodology to detect egg white in art samples

Michela Gambino, Francesca Cappitelli, Cristina Cattò, Aristodemo Carpen, more

Journal of Biosciences > 2013 > 38 > 2 > 397-408

A protocol for a simple and reliable dot-blot immunoassay was developed and optimized to test work of art samples for the presence of specific proteinaceus material (i.e. ovalbumin-based). The analytical protocol has been extensively set up with respect, among the other, to protein extraction conditions, to densitometric analysis and to the colorimetric reaction conditions. Feasibility evaluation...

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The constituents of the ink from a Qumran inkwell: new prospects for provenancing the ink on the Dead Sea Scrolls

Kaare Lund Rasmussen, Anna Lluveras Tenorio, Ilaria Bonaduce, Maria Perla Colombini, more

Journal of Archaeological Science > 2012 > 39 > 9 > 2956-2968

A unique sample of ink from an inkwell in the Schøyen Collection allegedly found at Qumran has been subjected to analyses by several analytical techniques: GC–MS, proteomic analysis, PXRD, Raman, (ATR) FT-IR, LIBS, ICP-MS and MS. The results reveal to an unexpected level of detail how the ink was manufactured, which gives insight into the industrial processes and craftsmanship that were practiced...

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Characterization of laccase isoforms produced by Pleurotus ostreatus in solid state fermentation of sugarcane bagasse

Susan Grace Karp, Vincenza Faraco, Antonella Amore, Leila Birolo, more

Bioresource Technology > 2012 > 114 > Complete > 735-739

Laccases are oxidative enzymes linked to biological degradation of lignin. The aim of this work was to evaluate the effect of inducers and different concentrations of nitrogen on production level of total laccase activity and pattern of laccase isoforms, produced in solid state fermentation of sugarcane bagasse by a selected strain of Pleurotus ostreatus. The addition of yeast extract 5g/L, copper...

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A family GH51 α-l-arabinofuranosidase from Pleurotus ostreatus : identification, recombinant expression and characterization

Antonella Amore, Angela Amoresano, Leila Birolo, Bernard Henrissat, more

Applied Microbiology and Biotechnology > 2012 > 94 > 4 > 995-1006

An α-l-arabinofuranosidase produced by Pleurotus ostreatus (PoAbf) during solid state fermentation on tomato pomace was identified and the corresponding gene and cDNA were cloned and sequenced. Molecular analysis showed that the poabf gene carries 26 exons interrupted by 25 introns and has an open reading frame encoding a protein of 646 amino acid residues, including a signal peptide of 20 amino acid...

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Resolution of the effects induced by W → F substitutions on the conformation and dynamics of the amyloid-forming apomyoglobin mutant W7FW14F

Giuseppe Infusini, Clara Iannuzzi, Silvia Vilasi, Leila Birolo, more

European Biophysics Journal > 2012 > 41 > 7 > 615-627

Myoglobin is an alpha-helical globular protein containing two highly conserved tryptophanyl residues at positions 7 and 14 in the N-terminal region. The simultaneous substitution of the two residues increases the susceptibility of the polypeptide chain to misfold, causing amyloid aggregation under physiological condition, i.e., neutral pH and room temperature. The role played by tryptophanyl residues...

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Fungal solid state fermentation on agro-industrial wastes for acid wastewater decolorization in a continuous flow packed-bed bioreactor

Donata Iandolo, Antonella Amore, Leila Birolo, Gabriella Leo, more

Bioresource Technology > 2011 > 102 > 16 > 7603-7607

This study was aimed at developing a process of solid state fermentation (SSF) with the fungi Pleurotus ostreatus and Trametes versicolor on apple processing residues for wastewater decolorization. Both fungi were able to colonize apple residues without any addition of nutrients, material support or water. P. ostreatus produced the highest levels of laccases (up to 9Ug ⁻¹ of dry matter) and...

article

Deamidation at Asparagine and Glutamine As a Major Modification upon Deterioration/Aging of Proteinaceous Binders in Mural Paintings

Gabriella Leo, Ilaria Bonaduce, Alessia Andreotti, Gennaro Marino, more

Analytical Chemistry > 2011 > 83 > 6 > 2056 - 2064

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PHK from phenol hydroxylase of Pseudomonas sp. OX1. Insight into the role of an accessory protein in bacterial multicomponent monooxygenases

Viviana Izzo, Gabriella Leo, Roberta Scognamiglio, Luca Troncone, more

Archives of Biochemistry and Biophysics > 2011 > 505 > 1 > 48-59

Bacterial multicomponent monooxygenases (BMMs) are members of a wide family of diiron enzymes that use molecular oxygen to hydroxylate a variety of aromatic compounds. The presence of genes encoding for accessory proteins not involved in catalysis and whose role is still elusive, is a common feature of the gene clusters of several BMMs, including phenol hydroxylases and several soluble methane monooxygenases...

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Stoichiometry and Topology of the Complex of the Endogenous ATP Synthase Inhibitor Protein IF ₁ with Calmodulin

Daniela Pagnozzi, Leila Birolo, Gabriella Leo, Stefania Contessi, more

Biochemistry > 2010 > 49 > 35 > 7542 - 7552

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Search results for: Leila Birolo

Evolved Gas Analysis‐Mass Spectrometry to Identify the Earliest Organic Binder in Aegean Style Wall Paintings

Evolved Gas Analysis‐Mass Spectrometry to Identify the Earliest Organic Binder in Aegean Style Wall Paintings

RHA-P: Isolation, expression and characterization of a bacterial α-l-rhamnosidase from Novosphingobium sp. PP1Y

A hypothesis on different technological solutions for outdoor and indoor Roman wall paintings

Regulating levels of the neuromodulator d‐serine in human brain: structural insight into pLG72 and d‐amino acid oxidase interaction

Proteomic strategies for cultural heritage: From bones to paintings

Femtosecond UV-laser pulses to unveil protein–protein interactions in living cells

Application of a new xylanase activity from Bacillus amyloliquefaciens XR44A in brewer's spent grain saccharification

Reply to Ira Rabin's Comment on our paper Rasmussen et al. (2012)

Ultraviolet laser‐induced cross‐linking in peptides

Industrial waste based compost as a source of novel cellulolytic strains and enzymes

A simple and reliable methodology to detect egg white in art samples

The constituents of the ink from a Qumran inkwell: new prospects for provenancing the ink on the Dead Sea Scrolls

Characterization of laccase isoforms produced by Pleurotus ostreatus in solid state fermentation of sugarcane bagasse

A family GH51 α-l-arabinofuranosidase from Pleurotus ostreatus : identification, recombinant expression and characterization

Resolution of the effects induced by W → F substitutions on the conformation and dynamics of the amyloid-forming apomyoglobin mutant W7FW14F

Fungal solid state fermentation on agro-industrial wastes for acid wastewater decolorization in a continuous flow packed-bed bioreactor

Deamidation at Asparagine and Glutamine As a Major Modification upon Deterioration/Aging of Proteinaceous Binders in Mural Paintings

PHK from phenol hydroxylase of Pseudomonas sp. OX1. Insight into the role of an accessory protein in bacterial multicomponent monooxygenases

Stoichiometry and Topology of the Complex of the Endogenous ATP Synthase Inhibitor Protein IF ₁ with Calmodulin

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