Search results for: Julie Audet

Items from 1 to 8 out of 8 results

article

Establishment of an erythroid progenitor cell line capable of enucleation achieved with an inducible c-Myc vector

Steven Mayers, Pablo Diego Moço, Talha Maqbool, Pamuditha N. Silva, more

BMC Biotechnology > 2019 > 19 > 1 > 1-13

Background A robust scalable method for producing enucleated red blood cells (RBCs) is not only a process to produce packed RBC units for transfusion but a potential platform to produce modified RBCs with applications in advanced cellular therapy. Current strategies for producing RBCs have shortcomings in the limited self-renewal capacity of progenitor cells, or difficulties in effectively enucleating...

chapter

Single-Cell Approaches to Dissect Cellular Signaling Networks

Weijia Wang, Julie Audet

Stem Cell Biology and Regenerative Medicine > Regulatory Networks in Stem Cells > Signaling and Regulation in Select Stem Cell Types > 337-345

Progress in understanding signal transduction, especially in rare and heterogeneous stem cell populations, is dependent on advances in single-cell assays. Newly developed techniques based on flow cytometry, capillary electrophoresis and live-cell imaging have enabled researchers to study kinase activity at the single-cell level. Since kinase activation is central to the regulation of virtually all...

article

Measurement of generation‐dependent proliferation rates and death rates during mouse erythroid progenitor cell differentiation

Vahe Akbarian, Weijia Wang, Julie Audet

Cytometry Part A > 81A > 5 > 382 - 389

Herein, we describe an experimental and computational approach to perform quantitative carboxyfluorescein diacetate succinimidyl ester (CFSE) cell‐division tracking in cultures of primary colony‐forming unit‐erythroid (CFU‐E) cells, a hematopoietic progenitor cell type, which is an important target for the treatment of blood disorders and for the manufacture of red blood cells. CFSE labeling of CFU‐Es...

article

Inhibition of glycogen synthase kinase-3 enhances the differentiation and reduces the proliferation of adult human olfactory epithelium neural precursors

Aziza P. Manceur, Michael Tseng, Tamara Holowacz, Ian Witterick, more

Experimental Cell Research > 2011 > 317 > 15 > 2086-2098

The olfactory epithelium (OE) contains neural precursor cells which can be easily harvested from a minimally invasive nasal biopsy, making them a valuable cell source to study human neural cell lineages in health and disease. Glycogen synthase kinase-3 (GSK-3) has been implicated in the etiology and treatment of neuropsychiatric disorders and also in the regulation of murine neural precursor cell...

article

Adventures in time and space: Nonlinearity and complexity of cytokine effects on stem cell fate decisions

Julie Audet

Biotechnology and Bioengineering > 106 > 2 > 173 - 182

Cytokines are central factors in the control of stem cell fate decisions and, as such, they are invaluable to those interested in the manipulation of stem and progenitor cells for clinical or research purposes. In their in vivo niches or in optimized cultures, stem cells are exposed to multiple cytokines, matrix proteins and other cell types that provide individual and combinatorial signals that influence...

article

Single amino acid resolution of proteolytic fragments generated in individual cells

Robert B. Brown, Johannes A. Hewel, Andrew Emili, Julie Audet

Cytometry Part A > 77A > 4 > 347 - 355

The complex nature of enzyme regulation mandates that enzyme activity profiles be measured in the context of the intact cell. Single‐cell capillary electrophoresis (CE) coupled with laser‐induced fluorescence is a powerful approach for quantitation and separation of analytes present in small samples and single live cells; however, it does not allow for the definitive identification of the reaction...

article

Flow cytometric screening of cell-penetrating peptides for their uptake into embryonic and adult stem cells

Aziza Manceur, Alice Wu, Julie Audet

Analytical Biochemistry > 2007 > 364 > 1 > 51-59

There is an increasing appreciation of the potential of cell-penetrating peptides (CPPs) as vectors to deliver peptides, proteins, and DNA into cells. However, the absolute and relative efficacy of various CPPs for applications targeting stem cells and primary cells is unclear. In this study, we have developed a two-step loading method and a flow cytometric assay to systematically compare the cellular...

article

Advances in hematopoietic stem cell culture

Julie Audet, Peter W Zandstra, Connie J Eaves, James M Piret

Current Opinion in Biotechnology > 1998 > 9 > 2 > 146-151

Recent advances in our understanding of the earliest stages of hematopoietic cell differentiation, and how these may be manipulated under defined conditions in vitro, have set the stage for the development of robust bioprocess technology applicable to hematopoietic cells. Sensitive and specific assays now exist for measuring the frequency of hematopoietic stem cells with long-term in vivo repopulating...

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Search results for: Julie Audet

Establishment of an erythroid progenitor cell line capable of enucleation achieved with an inducible c-Myc vector

Single-Cell Approaches to Dissect Cellular Signaling Networks

Measurement of generation‐dependent proliferation rates and death rates during mouse erythroid progenitor cell differentiation

Inhibition of glycogen synthase kinase-3 enhances the differentiation and reduces the proliferation of adult human olfactory epithelium neural precursors

Adventures in time and space: Nonlinearity and complexity of cytokine effects on stem cell fate decisions

Single amino acid resolution of proteolytic fragments generated in individual cells

Flow cytometric screening of cell-penetrating peptides for their uptake into embryonic and adult stem cells

Advances in hematopoietic stem cell culture

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