Vlad Zabrouskov

article

STORI Plots Enable Accurate Tracking of Individual Ion Signals

Jared O. Kafader, Steven C. Beu, Bryan P. Early, Rafael D. Melani, more

Journal of The American Society for Mass Spectrometry > 2019 > 30 > 11 > 2200-2203

Charge detection mass spectrometry (CDMS) of low-level signals is currently limited to the analysis of individual ions that generate a persistent signal during the entire observation period. Ions that disintegrate during the observation period produce reduced frequency domain signal amplitudes, which lead to an underestimation of the ion charge state, and thus the ion mass. The charge assignment can...

chapter

Analysis of Glycopeptides Using Porous Graphite Chromatography and LTQ Orbitrap XL ETD Hybrid MS

Terry Zhang, Rosa Viner, Zhiqi Hao, Vlad Zabrouskov

Sample Preparation in Biological Mass Spectrometry > Affinity Interaction and Biochemical Enrichment Techniques > 535-546

In this study, several glycoproteins: bovine α1-acid glycoprotein, fetuin, and human α1-acid glycoprotein, were analyzed using nanoLC-MS/MS. The performance of three different stationary phases – C₈, C₁₈, and porous graphite – was systematically evaluated and optimized for glycopeptide separation. A porous graphite column was chosen as the optimum stationary phase for glycoprotein analysis. An LTQ...

article

Enhanced Dissociation of Intact Proteins with High Capacity Electron Transfer Dissociation

Nicholas M. Riley, Christopher Mullen, Chad R. Weisbrod, Seema Sharma, more

Journal of The American Society for Mass Spectrometry > 2016 > 27 > 3 > 520-531

Electron transfer dissociation (ETD) is a valuable tool for protein sequence analysis, especially for the fragmentation of intact proteins. However, low product ion signal-to-noise often requires some degree of signal averaging to achieve high quality MS/MS spectra of intact proteins. Here we describe a new implementation of ETD on the newest generation of quadrupole-Orbitrap-linear ion trap Tribrid,...

article

Increased expression of lysosome membrane protein 2 in glomeruli of patients with idiopathic membranous nephropathy

Ilse M. Rood, Michael L. Merchant, Daniel W. Wilkey, Terry Zhang, more

PROTEOMICS > 15 > 21 > 3722 - 3730

Urinary microvesicles constitute a rich source of membrane‐bound and intracellular proteins that may provide important clues of pathophysiological mechanisms in renal disease. In the current study, we analyzed and compared the proteome of urinary microvesicles from patients with idiopathic membranous nephropathy (iMN), idiopathic focal segmental glomerulosclerosis (iFSGS), and normal controls using...

article

Micro-Heterogeneity of Human Saliva Peptide P-C Characterized by High-Resolution Top-Down Fourier-Transform Mass Spectrometry

Frédéric Halgand, Vlad Zabrouskov, Sara Bassilian, Puneet Souda, more

Journal of the American Society for Mass Spectrometry > 2010 > 21 > 5 > 868-877

Top-down proteomics characterizes protein primary structures with unprejudiced descriptions of expressed and processed gene products. Gene sequence polymorphisms, protein post-translational modifications, and gene sequence errors can all be identified using top-down proteomics. Saliva offers advantages for proteomic research because of availability and the noninvasiveness of collection and, for these...

article

Micro-heterogeneity of human saliva peptide P-C characterized by high-resolution top-down fourier-transform mass spectrometry

Frédéric Halgand, Vlad Zabrouskov, Sara Bassilian, Puneet Souda, more

Journal of The American Society for Mass Spectrometry > 2010 > 21 > 5 > 868-877

Top-down proteomics characterizes protein primary structures with unprejudiced descriptions of expressed and processed gene products. Gene sequence polymorphisms, protein post-translational modifications, and gene sequence errors can all be identified using top-down proteomics. Saliva offers advantages for proteomic research because of availability and the noninvasiveness of collection and, for these...

article

Dual-Pressure Linear Ion Trap Mass Spectrometer Improving the Analysis of Complex Protein Mixtures

Tonya Pekar Second, Justin D. Blethrow, Jae C. Schwartz, Gennifer E. Merrihew, more

Analytical Chemistry > 2009 > 81 > 18 > 7757 - 7765

article

Mass Measurement and Top-Down HPLC/MS Analysis of Intact Monoclonal Antibodies on a Hybrid Linear Quadrupole Ion Trap–Orbitrap Mass Spectrometer

Pavel V. Bondarenko, Tonya P. Second, Vlad Zabrouskov, Alexander A. Makarov, more

Journal of the American Society for Mass Spectrometry > 2009 > 20 > 8 > 1415-1424

Mass and top-down analyses of 150-kDa monoclonal immunoglobulin gamma (IgG) antibodies were performed on an Orbitrap analyzer. Three different sample delivery methods were tested including (1) infusion of an off-line desalted IgG sample using nano-electrospray; (2) on-line desalting followed by a step elution with a high percentage of organic solvent; and (3) reversed-phase HPLC separation and on-line...

article

Quantification of post-translationally modified peptides of bovine α-crystallin using tandem mass tags and electron transfer dissociation

Rosa I. Viner, Terry Zhang, Tonya Second, Vlad Zabrouskov

Journal of Proteomics > 2009 > 72 > 5 > 874-885

The modification of Ser/Thr residues in proteins by addition of single O-linked N-acetylglucosamine (O-GlcNAc) moieties play an important role in cell regulation. However, understanding the cellular mechanisms that regulate O-GlcNAc glycosylation has been challenging due to the difficulty in detection and quantification of this modification. Mass spectrometry-based multiplex quantitative approaches...

article

Mass measurement and top-down HPLC/MS analysis of intact monoclonal antibodies on a hybrid linear quadrupole ion trap-orbitrap mass spectrometer

Pavel V. Bondarenko, Tonya P. Second, Vlad Zabrouskov, Alexander A. Makarov, more

Journal of The American Society for Mass Spectrometry > 2009 > 20 > 8 > 1415-1424

Mass and top-down analyses of 150-kDa monoclonal immunoglobulin gamma (IgG) antibodies were performed on an Orbitrap analyzer. Three different sample delivery methods were tested including (1) infusion of an off-line desalted IgG sample using nano-electrospray; (2) on-line desalting followed by a step elution with a high percentage of organic solvent; and (3) reversed-phase HPLC separation and on-line...

article

A Proteomics Grade Electron Transfer Dissociation-Enabled Hybrid Linear Ion Trap-Orbitrap Mass Spectrometer

Graeme C. McAlister, W. Travis Berggren, Jens Griep-Raming, Stevan Horning, more

Journal of Proteome Research > 2008 > 7 > 8 > 3127 - 3136

article

Protein-sequence polymorphisms and post-translational modifications in proteins from human saliva using top-down Fourier-transform ion cyclotron resonance mass spectrometry

Julian P. Whitelegge, Vlad Zabrouskov, Frederic Halgand, Puneet Souda, more

International Journal of Mass Spectrometry > 2007 > 268 > 2-3 > 190-197

Single nucleotide polymorphisms (SNPs) can result in protein-sequence polymorphisms (PSPs) when codon translations are altered. Both top-down and bottom-up proteomics strategies can identify PSPs, but only if databases and software are used with this in mind. A 14,319Da protein from human saliva was characterized using the top-down approach on a hybrid linear ion-trap Fourier-transform ion cyclotron...

article

Increased Coverage in the Transmembrane Domain with Activated-Ion Electron Capture Dissociation for Top-Down Fourier-Transform Mass Spectrometry of Integral Membrane Proteins

Vlad Zabrouskov, Julian P. Whitelegge

Journal of Proteome Research > 2007 > 6 > 6 > 2205 - 2210

article

Performance of a Linear Ion Trap-Orbitrap Hybrid for Peptide Analysis

John R. Yates, Daniel Cociorva, Lujian Liao, Vlad Zabrouskov

Analytical Chemistry > 2006 > 78 > 2 > 493 - 500

article

Stepwise Deamidation of Ribonuclease A at Five Sites Determined by Top Down Mass Spectrometry ^†

Vlad Zabrouskov, Xuemei Han, Ervin Welker, Huili Zhai, more

Biochemistry > 2006 > 45 > 3 > 987 - 992

article

New and Automated MSn Approaches for Top-Down Identification of Modified Proteins

Vlad Zabrouskov, Michael W. Senko, Yi Du, Richard D. Leduc, more

Journal of the American Society for Mass Spectrometry > 2005 > 16 > 12 > 2027-2038

An automated top-down approach including data-dependent MS ³ experiment for protein identification/characterization is described. A mixture of wild-type yeast proteins has been separated on-line using reverse-phase liquid chromatography and introduced into a hybrid linear ion trap (LTQ) Fourier transform ion cylclotron resonance (FTICR) mass spectrometer, where the most abundant molecular...

article

New and automated MSⁿ approaches for top-down identification of modified proteins

Vlad Zabrouskov, Michael W. Senko, Yi Du, Richard D. Leduc, more

Journal of The American Society for Mass Spectrometry > 2005 > 16 > 12 > 2027-2038

An automated top-down approach including data-dependent MS³ experiment for protein identification/characterization is described. A mixture of wild-type yeast proteins has been separated on-line using reverse-phase liquid chromatography and introduced into a hybrid linear ion trap (LTQ) Fourier transform ion cylclotron resonance (FTICR) mass spectrometer, where the most abundant molecular ions were...

INFONA - science communication portal

Search results for: Vlad Zabrouskov

STORI Plots Enable Accurate Tracking of Individual Ion Signals

Analysis of Glycopeptides Using Porous Graphite Chromatography and LTQ Orbitrap XL ETD Hybrid MS

Enhanced Dissociation of Intact Proteins with High Capacity Electron Transfer Dissociation

Increased expression of lysosome membrane protein 2 in glomeruli of patients with idiopathic membranous nephropathy

Micro-Heterogeneity of Human Saliva Peptide P-C Characterized by High-Resolution Top-Down Fourier-Transform Mass Spectrometry

Micro-heterogeneity of human saliva peptide P-C characterized by high-resolution top-down fourier-transform mass spectrometry

Dual-Pressure Linear Ion Trap Mass Spectrometer Improving the Analysis of Complex Protein Mixtures

Mass Measurement and Top-Down HPLC/MS Analysis of Intact Monoclonal Antibodies on a Hybrid Linear Quadrupole Ion Trap–Orbitrap Mass Spectrometer

Quantification of post-translationally modified peptides of bovine α-crystallin using tandem mass tags and electron transfer dissociation

Mass measurement and top-down HPLC/MS analysis of intact monoclonal antibodies on a hybrid linear quadrupole ion trap-orbitrap mass spectrometer

A Proteomics Grade Electron Transfer Dissociation-Enabled Hybrid Linear Ion Trap-Orbitrap Mass Spectrometer

Protein-sequence polymorphisms and post-translational modifications in proteins from human saliva using top-down Fourier-transform ion cyclotron resonance mass spectrometry

Increased Coverage in the Transmembrane Domain with Activated-Ion Electron Capture Dissociation for Top-Down Fourier-Transform Mass Spectrometry of Integral Membrane Proteins

Performance of a Linear Ion Trap-Orbitrap Hybrid for Peptide Analysis

Stepwise Deamidation of Ribonuclease A at Five Sites Determined by Top Down Mass Spectrometry ^†

New and Automated MSn Approaches for Top-Down Identification of Modified Proteins

New and automated MSⁿ approaches for top-down identification of modified proteins

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