Search results for: Zenon J. Kokot

Items from 1 to 20 out of 26 results

article

Proteomic features characterization of Hymenoptera venom allergy

Eliza Matuszewska, Joanna Matysiak, Anna Bręborowicz, Katarzyna Olejniczak, more

Allergy, Asthma & Clinical Immunology > 2019 > 15 > 1 > 1-8

Background Hymenoptera venom allergy is one of the most frequent causes of anaphylaxis. In its most severe form, the reaction to wasp and honey bee stings may be life-threatening. Therefore, immediate and proper diagnosis of venom allergy and implementation of suitable therapy are extremely important. Broadening the knowledge on the mechanism of the allergic reaction may contribute to the improvement...

article

Serum lipidome screening in patients with stage I non-small cell lung cancer

Agnieszka Klupczynska, Szymon Plewa, Mariusz Kasprzyk, Wojciech Dyszkiewicz, more

Clinical and Experimental Medicine > 2019 > 19 > 4 > 505-513

The ability of early lung cancer diagnosis is an unmet need in clinical practice. Lung cancer metabolomic analyses conducted so far have demonstrated several abnormalities in cancer lipid profile providing a rationale for further study of blood lipidome of the patients. In the present research, we performed a targeted lipidome screening to select molecules that show promise for early lung cancer detection...

article

Mass spectrometry-based proteomics techniques and their application in ovarian cancer research

Agata Swiatly, Szymon Plewa, Jan Matysiak, Zenon J. Kokot

Journal of Ovarian Research > 2018 > 11 > 1 > 1-13

Ovarian cancer has emerged as one of the leading cause of gynecological malignancies. So far, the measurement of CA125 and HE4 concentrations in blood and transvaginal ultrasound examination are essential ovarian cancer diagnostic methods. However, their sensitivity and specificity are still not sufficient to detect disease at the early stage. Moreover, applied treatment may appear to be ineffective...

article

A study of low-molecular-weight organic acid urinary profiles in prostate cancer by a new liquid chromatography-tandem mass spectrometry method

Agnieszka Klupczynska, Szymon Plewa, Natalia Sytek, Wojciech Sawicki, more

Journal of Pharmaceutical and Biomedical Analysis > 2018 > 159 > C > 229-236

Metabolomic studies constantly require high throughput screenings, and this drives development and optimization of methods that include more analytes in a single run, shorten the analysis time and simplify sample preparation. The aim of the study was to develop a new simple and fast liquid chromatography-tandem mass spectrometry-based methodology for quantitative analysis of a panel of ten organic...

article

The concomitant use of lapatinib and paracetamol - the risk of interaction

Agnieszka Karbownik, Edyta Szałek, Katarzyna Sobańska, Tomasz Grabowski, more

Investigational New Drugs > 2018 > 36 > 5 > 819-827

Summary Lapatinib is a tyrosine kinase inhibitor used for the treatment of breast cancer. Paracetamol is an analgesic commonly applied to patients with mild or moderate pain and fever. Cancer patients are polymedicated, which involves high risk of drug interactions during therapy. The aim of the study was to assess the interaction between lapatinib and paracetamol in rats. The rats were divided into...

article

Development and validation of HPLC–MS/MS procedure for determination of 3,4,4′,5-tetra-methoxystilbene (DMU-212) and its metabolites in ovarian cancer cells and culture medium

Agnieszka Klupczynska, Anna Maria Sulej-Suchomska, Hanna Piotrowska-Kempisty, Marcin Wierzchowski, more

Journal of Chromatography B > 2017 > 1060 > C > 30-35

The synthetic resveratrol analogue DMU-212 (3,4,4′,5-tetramethoxystilbene) has been shown to possess stronger anticancer activity than resveratrol in a variety of tumour cells. To date, there has been no appropriate procedure that would ensure a reliable data about levels of metabolic products of DMU-212 in cancer cell lines. The purpose of this study was to develop a new procedure for determination...

article

Mass spectrometry as a tool for biomarkers searching in gynecological oncology

Paulina Banach, Wiktor Suchy, Paweł Dereziński, Jan Matysiak, more

Biomedicine & Pharmacotherapy > 2017 > 92 > C > 836-842

Tumors of the female reproductive tract are an important target for the development of diagnostic, prognostic and therapeutic strategies. Recent research has turned to proteomics based on mass spectrometry techniques, to achieve more effective diagnostic results. Mass spectrometry (MS) enables identification and quantification of multiple molecules simultaneously in a single experiment according to...

article

MALDI-TOF-MS analysis in discovery and identification of serum proteomic patterns of ovarian cancer

Agata Swiatly, Agnieszka Horala, Joanna Hajduk, Jan Matysiak, more

BMC Cancer > 2017 > 17 > 1 > 1-9

Background Due to high mortality and lack of efficient screening, new tools for ovarian cancer (OC) diagnosis are urgently needed. To broaden the knowledge on the pathological processes that occur during ovarian cancer tumorigenesis, protein-peptide profiling was proposed. Methods Serum proteomic patterns in samples from OC patients were obtained using matrix-assisted laser desorption/ionization...

article

The feature selection approach for evaluation of potential rheumatoid arthritis markers using MALDI-TOF datasets

Bartosz Urbaniak, Piotr Nowicki, Dorota Sikorska, Włodzimierz Samborski, more

Analytical Biochemistry > 2017 > 525 > C > 29-37

The selection of the most representative mass profiles, in rheumatoid arthritis (RA) serum samples was developed. This allows for selection and identification of potential biomarkers in RA serum samples.The RA and controls samples were analyzed using MALDI-TOF. Two different protein elution procedures utilizing ZipTips (E1 and E2) were examined. The statistical evaluation of data was performed using...

article

Determination of 16 serum angiogenic factors in stage I non-small cell lung cancer using a bead-based multiplex immunoassay

Agnieszka Klupczynska, Paweł Dereziński, Jan Matysiak, Wojciech Dyszkiewicz, more

Biomedicine & Pharmacotherapy > 2017 > 88 > C > 1031-1037

The aim of the study was to identify significant abnormalities in angiogenic factor profiles occurring at early-stage non-small cell lung cancer (NSCLC). Contrary to the previous studies, our research included patients with only stage I NSCLC, which allowed for estimating the utility of circulating angiogenic factors in early NSCLC detection. The investigation was performed in serum samples collected...

article

Study of early stage non-small-cell lung cancer using Orbitrap-based global serum metabolomics

Agnieszka Klupczynska, Paweł Dereziński, Timothy J. Garrett, Vanessa Y. Rubio, more

Journal of Cancer Research and Clinical Oncology > 2017 > 143 > 4 > 649-659

Purpose The aim of the project was to apply ultra-high-performance liquid chromatography–quadrupole-Orbitrap-high-resolution mass spectrometry for serum metabolite profiling of non-small-cell lung cancer (NSCLC). This Orbitrap-based methodology has been applied for a study of NSCLC potential markers for the first time. Methods After extraction using protein precipitation, sera were separated on...

article

Alergia na jad pszczeli u pszczelarzy

Joanna Matysiak, Zenon J. Kokot, Jan Matysiak

Alergia Astma Immunologia - przegląd kliniczny > 2017 > 22 > 1 > 5-11

Pszczelarze są szczególną grupą osób najbardziej narażonych na użą- dlenia i alergię na jad pszczeli z racji wykonywanej pracy. Dodatnie wyniki testów skórnych lub obecność swoistych dla jadu pszczelego IgE obserwowane są nawet u 60% badanych pszczelarzy. Reakcja po użądleniu przez pszczołę najczęściej występuje pod postacią normalnej reakcji miejscowej, ale u osoby uczulonej na jad pszczeli może...

article

Evaluation of serum amino acid profiles’ utility in non-small cell lung cancer detection in Polish population

Agnieszka Klupczynska, Paweł Dereziński, Wojciech Dyszkiewicz, Krystian Pawlak, more

Lung Cancer > 2016 > 100 > C > 71-76

Data from studies performed in Japanese and Korean populations suggest that free amino acid profiles have the potential to aid in non-small cell lung cancer (NSCLC) detection. However, there is still no data regarding abnormalities of free amino acids and their usefulness in NSCLC detection in European populations. The aim of the study was an evaluation of utility of amino acid profiles in NSCLC detection...

article

Determination of low-molecular-weight organic acids in non-small cell lung cancer with a new liquid chromatography–tandem mass spectrometry method

Agnieszka Klupczynska, Szymon Plewa, Wojciech Dyszkiewicz, Mariusz Kasprzyk, more

Journal of Pharmaceutical and Biomedical Analysis > 2016 > 129 > C > 299-309

As compared to other classes of metabolites, determination of organic acids is an underrepresented field in cancer research and till now there has been a lack of appropriate analytical procedure for determination of serum levels of organic acids potentially associated with cancer development. The aim of the study was to develop a new rapid liquid chromatography–tandem mass spectrometry method for...

article

Challenges in biomarker discovery with MALDI-TOF MS

Joanna Hajduk, Jan Matysiak, Zenon J. Kokot

Clinica Chimica Acta > 2016 > 458 > Complete > 84-98

MALDI-TOF MS technique is commonly used in system biology and clinical studies to search for new potential markers associated with pathological conditions. Despite numerous concerns regarding a sample preparation or processing of complex data, this strategy is still recognized as a popular tool and its awareness has risen in the proteomic community over the last decade. In this review, we present...

article

Hyphenated LC–MALDI–ToF/ToF and LC–ESI–QToF approach in proteomic characterization of honeybee venom

Jan Matysiak, Joanna Hajduk, Franz Mayer, Romano Hebeler, more

Journal of Pharmaceutical and Biomedical Analysis > 2016 > 121 > C > 69-76

To increase in the depth characterization of venom proteome of Apis mellifera the hyphenated LC–MALDI–ToF/ToF–MS (liquid chromatography–matrix-assisted laser desorption/ionization–time of flight/time of flight tandem mass spectrometry) and LC–ESI–QToF–MS (liquid chromatography–electrospray ionization–quadrupole time of flight tandem mass spectrometry) techniques combined with combinatorial peptide...

article

Estimation of drug abuse in 9 Polish cities by wastewater analysis

Agnieszka Klupczynska, Paweł Dereziński, Janusz Krysztofiak, Zenon J. Kokot

Forensic Science International > 2016 > 260 > C > 14-21

The aim of this work was to measure illicit drug residues in raw sewage samples collected from nine Polish cities in order to determine trends in illicit drug use in these urban populations. This is the first study involving an analysis of samples from several sewage treatment plants in Poland and covering such a large population. Concentration of illicit drugs was determined using a high performance...

article

The application of fuzzy statistics and linear discriminant analysis as criteria for optimizing the preparation of plasma for matrix-assisted laser desorption/ionization mass spectrometry peptide profiling

Joanna Hajduk, Jan Matysiak, Piotr Kokot, Piotr Nowicki, more

Clinica Chimica Acta > 2015 > 448 > Complete > 174-181

An alternative bioinformatics approach based on fuzzy theory statistics and linear discriminant analysis is proposed for the interpretation of MALDI MS spectra in peptide profiling. When applied, the methodology enables the establishment of a reproducible plasma preparation protocol appropriate for the evaluation of small data sets. The samples were collected from pregnant women affected by gestational...

article

Shotgun proteome analysis of honeybee venom using targeted enrichment strategies

Jan Matysiak, Joanna Hajduk, Łukasz Pietrzak, Christian E.H. Schmelzer, more

Toxicon > 2014 > 90 > Complete > 255-264

The aim of this study was to explore the honeybee venom proteome applying a shotgun proteomics approach using different enrichment strategies (combinatorial peptide ligand libraries and solid phase extraction). The studies were conducted using nano-LC/MALDI-TOF/TOF-MS system. The MS analysis of peptide profiles (in the range of 900–4500 Da) and virtual gel-image of proteins from Lab-on-Chip assay...

article

A new method for determination of hyaluronidase activity in biological samples using capillary zone electrophoresis

Jan Matysiak, Paweł Dereziński, Bartosz Urbaniak, Agnieszka Klupczyńska, more

Biomedical Chromatography > 27 > 8 > 1070 - 1078

The aim of the study was to develop a new capillary zone electrophoresis (CZE) method for determination of enzymatic activity of hyaluronidase. The method permits monitoring of the process of hyaluronic acid digestion by hyaluronidase. Studies were performed using CZE instrument equipped with capillary of 64.5 cm total length, 56 cm effective length and internal diameter 75 µm. Separation was performed...

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Search results for: Zenon J. Kokot

Proteomic features characterization of Hymenoptera venom allergy

Serum lipidome screening in patients with stage I non-small cell lung cancer

Mass spectrometry-based proteomics techniques and their application in ovarian cancer research

A study of low-molecular-weight organic acid urinary profiles in prostate cancer by a new liquid chromatography-tandem mass spectrometry method

The concomitant use of lapatinib and paracetamol - the risk of interaction

Development and validation of HPLC–MS/MS procedure for determination of 3,4,4′,5-tetra-methoxystilbene (DMU-212) and its metabolites in ovarian cancer cells and culture medium

Mass spectrometry as a tool for biomarkers searching in gynecological oncology

MALDI-TOF-MS analysis in discovery and identification of serum proteomic patterns of ovarian cancer

The feature selection approach for evaluation of potential rheumatoid arthritis markers using MALDI-TOF datasets

Determination of 16 serum angiogenic factors in stage I non-small cell lung cancer using a bead-based multiplex immunoassay

Study of early stage non-small-cell lung cancer using Orbitrap-based global serum metabolomics

Alergia na jad pszczeli u pszczelarzy

Evaluation of serum amino acid profiles’ utility in non-small cell lung cancer detection in Polish population

Determination of low-molecular-weight organic acids in non-small cell lung cancer with a new liquid chromatography–tandem mass spectrometry method

Challenges in biomarker discovery with MALDI-TOF MS

Hyphenated LC–MALDI–ToF/ToF and LC–ESI–QToF approach in proteomic characterization of honeybee venom

Estimation of drug abuse in 9 Polish cities by wastewater analysis

The application of fuzzy statistics and linear discriminant analysis as criteria for optimizing the preparation of plasma for matrix-assisted laser desorption/ionization mass spectrometry peptide profiling

Shotgun proteome analysis of honeybee venom using targeted enrichment strategies

A new method for determination of hyaluronidase activity in biological samples using capillary zone electrophoresis

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