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We previously reported that cell death of rat spinal motoneurons, induced by trophic factor-deprivation, was attenuated by the application of exogenous cell-permeable ceramide (C 6 -Cer), or bacterial sphingomyelinase (SMase). Recently, motoneuronal cell death was demonstrated to be mediated by the generation of reactive oxygen species (ROS), including superoxide and peroxinitrite. In this...
To determine Fas-induced reactive oxygen species (ROS) generation in cells sensitive to Fas-mediated apoptosis, chemiluminescence, a strong indicator of ROS generation, was monitored after stimulation with agonistic anti-Fas. Fas ligation resulted in a rapid and sustained ROS generation. The generation reached a maximum within 5 min and was still observed at least 40 min after the stimulation. No...
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