Search results for: Maria Adelaide Pronzato

Items from 1 to 20 out of 27 results

article

Glutathione and the switch of aerobic metabolism collaborate for multi-drug resistance of neuroblastoma

Cinzia Domenicotti, Andrea Speciale, Ombretta Garbarino, Alberto Izzotti, more

Free Radical Biology and Medicine > 2017 > 108 > S1 > S69

The availability of antioxidants is recognized as one of the critical factors able to make cancer cells resistant to chemotherapy. In this context, it has been demonstrated that many chemoresistant cancers display high levels of glutathione (GSH) and consequently, its depletion by L-buthionine sulfoximine (BSO), has been proposed as a chemosensitizing therapy. To investigate the role of GSH and of...

article

New insights into selective PDE4D inhibitors: 3-(Cyclopentyloxy)-4-methoxybenzaldehyde O-(2-(2,6-dimethylmorpholino)-2-oxoethyl) oxime (GEBR-7b) structural development and promising activities to restore memory impairment

Chiara Brullo, Roberta Ricciarelli, Jos Prickaerts, Ottavio Arancio, more

European Journal of Medicinal Chemistry > 2016 > 124 > C > 82-102

Phosphodiesterase type 4D (PDE4D) has been indicated as a promising target for treating neurodegenerative pathologies such as Alzheimer's Disease (AD). By preventing cAMP hydrolysis, PDE4 inhibitors (PDE4Is) increase the cAMP response element-binding protein (CREB) phosphorylation, synaptic plasticity and long-term memory formation. Pharmacological and behavioral studies on our hit GEBR-7b demonstrated...

article

HO-1 Down-Regulation Increases the Efficacy of BRAFV600E Inhibition-Based Therapy in Primary Melanoma Cells

Anna Lisa Furfaro, Gabriella Pietra, Umberto Maria Marinari, Lorenzo Moretta, more

Free Radical Biology and Medicine > 2016 > 100 > S > S122

The inducible enzyme heme oxygenase 1 (HO-1) plays a crucial role in maintaining redox balance. Under stress conditions, HO-1 is up-regulated leading to the generation of bilirubin, ferritin and carbon monoxide, with antioxidant, antiapoptotic and anti-inflammatory properties [1]. The induction of HO-1 is involved in the gain of resistance to oxidation-based therapies and favors cancer progression...

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Neuroblastoma Cell Response to Oxidative Stress Is Impaired by Retinoic Acid-Induced Differentiation: Role of HO-1

Mariapaola Nitti, Sabrina Piras, Anna Lisa Furfaro, Lorenzo Brondolo, more

Free Radical Biology and Medicine > 2016 > 100 > S > S106-S107

The modulation of redox balance is able to drive different cell functions, from differentiation to proliferation. In a previous work, we showed that all-trans retinoic acid (ATRA) induced differentiation of SH-SY5Y neuroblastoma (NB) cells through the generation of reactive oxygen species (ROS) [Nitti et al Cell Signal 2010]. However, ATRA-differentiated NB cells resulted more sensitive to the further...

article

Oxysterol mixture and, in particular, 27‐hydroxycholesterol drive M2 polarization of human macrophages

Barbara Marengo, Francesca Bellora, Roberta Ricciarelli, Chiara De Ciucis, more

BioFactors > 42 > 1 > 80 - 92

Macrophages play a crucial role in atherosclerosis progression. Classically activated M1 macrophages have been found in rupture‐prone atherosclerotic plaques whereas alternatively activated macrophages, M2, localize in stable plaque. Macrophage accumulation of cholesterol and of its oxidized derivatives (oxysterols) leads to the formation of foam cells, a hallmark of atherosclerotic lesions. In this...

article

Heme oxygenase-1-derived bilirubin protects endothelial cells against high glucose-induced damage

Meihua He, Mariapaola Nitti, Sabrina Piras, Anna Lisa Furfaro, more

Free Radical Biology and Medicine > 2015 > 89 > C > 91-98

Hyperglycemia and diabetes are associated with endothelial cell dysfunction arising from enhanced oxidative injury, leading to the progression of diabetic vascular pathologies. The redox-sensitive transcription factor nuclear factor-E2-related factor 2 (Nrf2) is a master regulator of antioxidant genes, such as heme oxygenase-1 (HO-1), involved in cellular defenses against oxidative stress. We have...

article

Synthesis, biological activities and pharmacokinetic properties of new fluorinated derivatives of selective PDE4D inhibitors

Chiara Brullo, Matteo Massa, Carla Villa, Roberta Ricciarelli, more

Bioorganic & Medicinal Chemistry > 2015 > 23 > 13 > 3426-3435

A new series of selective PDE4D inhibitors has been designed and synthesized by replacing 3-methoxy group with 3-difluoromethoxy isoster moiety in our previously reported cathecolic structures. All compounds showed a good PDE4D3 inhibitory activity, most of them being inactive toward other PDE4 isoforms (PDE4A4, PDE4B2 and PDE4C2). Compound 3b, chosen among the synthesized compounds as the most promising...

article

A novel mechanism for cyclic adenosine monophosphate–mediated memory formation: Role of amyloid beta

Roberta Ricciarelli, Daniela Puzzo, Olga Bruno, Elisa Canepa, more

Annals of Neurology > 75 > 4 > 602 - 607

Cyclic adenosine monophosphate (cAMP) regulates long‐term potentiation (LTP) and ameliorates memory in healthy and diseased brain. Increasing evidence shows that, under physiological conditions, low concentrations of amyloid β (Aβ) are necessary for LTP expression and memory formation. Here, we report that cAMP controls amyloid precursor protein (APP) translation and Aβ levels, and that the modulatory...

article

HO-1 up-regulation: A key point in high-risk neuroblastoma resistance to bortezomib

Anna Lisa Furfaro, Sabrina Piras, Mario Passalacqua, Cinzia Domenicotti, more

BBA - Molecular Basis of Disease > 2014 > 1842 > 4 > 613-622

High-risk neuroblastoma (NB) is characterized by the development of chemoresistance, and bortezomib (BTZ), a selective inhibitor of proteasome, has been proposed in order to overcome drug resistance. Considering the involvement of the nuclear factor-erythroid-derived 2-like 2 (Nrf2) and heme oxygenase-1 (HO-1) in the antioxidant and detoxifying ability of cancer cells, in this study we have investigated...

article

Short exposure of albumin to high concentrations of malondialdehyde does not mimic physiological conditions

Susanna Millanta, Anna Lisa Furfaro, Paolo Carlier, Bruno Tasso, more

Experimental and Molecular Pathology > 2013 > 94 > 1 > 270-276

Malondialdehyde (MDA), a major lipid peroxidation product, spontaneously binds to, and modifies proteins. In vivo, proteins are physiologically exposed to micromolar MDA concentrations for long periods. In order to mimic this process in vitro, protein modification is often performed by short exposure to millimolar MDA concentrations, also in order to generate antigenic structures for antibody production...

article

Resistance of neuroblastoma GI-ME-N cell line to glutathione depletion involves Nrf2 and heme oxygenase-1

Anna Lisa Furfaro, José Raúl Zumba Macay, Barbara Marengo, Mariapaola Nitti, more

Free Radical Biology and Medicine > 2012 > 52 > 2 > 488-496

Cancer cell survival is known to be related to the ability to counteract oxidative stress, and glutathione (GSH) depletion has been proposed as a mechanism to sensitize cells to anticancer therapy. However, we observed that GI-ME-N cells, a neuroblastoma cell line without MYCN amplification, are able to survive even if GSH-depleted by l-buthionine-(S,R)-sulfoximine (BSO). Here, we show that in GI-ME-N...

article

PKC delta and NADPH oxidase in retinoic acid-induced neuroblastoma cell differentiation

Mariapaola Nitti, Anna Lisa Furfaro, Claudia Cevasco, Nicola Traverso, more

Cellular Signalling > 2010 > 22 > 5 > 828-835

The role of reactive oxygen species (ROS) in the regulation of signal transduction processes has been well established in many cell types and recently the fine tuning of redox signalling in neurons received increasing attention. With regard to this, the involvement of NADPH oxidase (NOX) in neuronal pathophysiology has been proposed but deserves more investigation. In the present study, we used SH-SY5Y...

article

A comparative study of leukaemia inhibitory factor and interleukin-1α intracellular content in a human keratinocyte cell line after exposure to cosmetic fragrances and sodium dodecyl sulphate

Alessandro Parodi, Roberta Sanguineti, Mariafrancesca Catalano, Susanna Penco, more

Toxicology Letters > 2010 > 192 > 2 > 101-107

According to European laws animal testing in cosmetic industry will be prohibited in a few years and it will be replaced by alternative methods based on cell and tissue culture. Many ingredients of cosmetic formulations are potentially causes of skin inflammation and sensibilization. Since cytotoxicity is known, among other factors, to trigger irritation, in an alternative model for evaluation of...

article

Heme oxygenase 1 expression in rat liver during ageing and ethanol intoxication

Stefania Patriarca, Anna Lisa Furfaro, Luana Cosso, Elena Pesce Maineri, more

Biogerontology > 2007 > 8 > 3 > 365-372

Heme oxygenase 1 (HO-1) expression is recognized as a marker of cellular response to oxidative stress; since ageing is believed to be related to oxidative “wear and tear”, HO-1 may represent a candidate biomarker of ageing. In our study, the hepatic expression of HO-1 mRNA, evaluated by RT-PCR in 2.5–24 month-old rats, was higher at 6 months than at 2.5 months of age, but thereafter increased no further:...

article

Reactive oxygen species: Biological stimuli of neuroblastoma cell response

Barbara Marengo, Lizzia Raffaghello, Vito Pistoia, Damiano Cottalasso, more

Cancer Letters > 2005 > 228 > 1-2 > 111-116

Reactive oxygen species play a critical role in differentiation, proliferation and apoptosis acting as ‘second messengers’ able to regulate sulphydryl groups in signaling molecules as protein kinase C, a family of isoenzymes involved in many cellular responses and implicated in cell transformation. Neuroblastoma is characterised by the production of oxygen intermediates and l-buthionine-S,R-sulfoximine,...

article

Role of phosphatidylinositol 3-kinase in the development of hepatocyte preconditioning

Rita Carini, Maria Grazia De Cesaris, Roberta Splendore, Gianluca Baldanzi, more

Gastroenterology > 2004 > 127 > 3 > 914-923

Background & Aims:Ischemic preconditioning has been proved effective in reducing ischemia/reperfusion injury during liver surgery. However, the mechanisms involved are still poorly understood. Here, we have investigated the role of phosphatidylinositol 3-kinase (PI3K) in the signal pathway leading to hepatic preconditioning. Methods:PI3K activation was evaluated in isolated rat hepatocytes preconditioned...

article

Anti malondialdehyde-adduct immunological response as a possible marker of successful aging

Nicola Traverso, Stefania Patriarca, Emanuela Balbis, Anna Lisa Furfaro, more

Experimental Gerontology > 2003 > 38 > 10 > 1129-1135

Contrasting results have been obtained by various researchers about oxidative markers of aging. In this study, a healthy over-90-year-old population was examined for various plasma oxidative biomarkers and compared with a healthy population of blood donors (age range 23-66). Plasma malondialdehyde (MDA), evaluated by means of the thiobarbituric acid test, was significantly higher in the over-90-year-old...

article

Role of PKC-δ activity in glutathione-depleted neuroblastoma cells

Cinzia Domenicotti, Barbara Marengo, Daniela Verzola, Giacomo Garibotto, more

Free Radical Biology and Medicine > 2003 > 35 > 5 > 504-516

Protein kinases C (PKCs) are a family of isoenzymes sensitive to oxidative modifications and involved in the transduction signal pathways that regulate cell growth. As such, they can act as cellular sensors able to intercept intracellular redox changes and promote the primary adaptive cell response. In this study, we have demonstrated that PKC isoforms are specifically influenced by the amount of...

article

Role of PKC-dependent pathways in HNE-induced cell protein transport and secretion

Umberto Maria Marinari, Mariapaola Nitti, Maria Adelaide Pronzato, Cinzia Domenicotti

Molecular Aspects of Medicine > 2003 > 24 > 4-5 > 205-211

The β isoforms of protein Kinase C (PKC) are closely involved in the regulation of cell protein transport and secretion. We have shown in different cellular types that treatment with HNE in a concentration range detectable in many pathophysiological conditions is able to induce selective activation of βPKCs through direct interaction between the aldehyde and these isoenzymes. In isolated rat hepatocytes...

article

Signal pathway responsible for hepatocyte preconditioning by nitric oxide

Rita Carini, Maria Grazia De Cesaris, Roberta Splendore, Cinzia Domenicotti, more

Free Radical Biology and Medicine > 2003 > 34 > 8 > 1047-1055

Nitric oxide (NO) improves liver resistance to hypoxia/reperfusion injury acting as a mediator of hepatic preconditioning. However, the mechanisms involved are still poorly understood. In this study, we have investigated the mechanisms by which short-term exposure to the NO donor (Z)-1-(N-methyl-N-[6-(N-methylammoniohexyl)amino])-diazen-1-ium-1,2-diolate (NOC-9) increases hepatocyte tolerance to hypoxic...

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Search results for: Maria Adelaide Pronzato

Glutathione and the switch of aerobic metabolism collaborate for multi-drug resistance of neuroblastoma

New insights into selective PDE4D inhibitors: 3-(Cyclopentyloxy)-4-methoxybenzaldehyde O-(2-(2,6-dimethylmorpholino)-2-oxoethyl) oxime (GEBR-7b) structural development and promising activities to restore memory impairment

HO-1 Down-Regulation Increases the Efficacy of BRAFV600E Inhibition-Based Therapy in Primary Melanoma Cells

Neuroblastoma Cell Response to Oxidative Stress Is Impaired by Retinoic Acid-Induced Differentiation: Role of HO-1

Oxysterol mixture and, in particular, 27‐hydroxycholesterol drive M2 polarization of human macrophages

Heme oxygenase-1-derived bilirubin protects endothelial cells against high glucose-induced damage

Synthesis, biological activities and pharmacokinetic properties of new fluorinated derivatives of selective PDE4D inhibitors

A novel mechanism for cyclic adenosine monophosphate–mediated memory formation: Role of amyloid beta

HO-1 up-regulation: A key point in high-risk neuroblastoma resistance to bortezomib

Short exposure of albumin to high concentrations of malondialdehyde does not mimic physiological conditions

Resistance of neuroblastoma GI-ME-N cell line to glutathione depletion involves Nrf2 and heme oxygenase-1

PKC delta and NADPH oxidase in retinoic acid-induced neuroblastoma cell differentiation

A comparative study of leukaemia inhibitory factor and interleukin-1α intracellular content in a human keratinocyte cell line after exposure to cosmetic fragrances and sodium dodecyl sulphate

Heme oxygenase 1 expression in rat liver during ageing and ethanol intoxication

Reactive oxygen species: Biological stimuli of neuroblastoma cell response

Role of phosphatidylinositol 3-kinase in the development of hepatocyte preconditioning

Anti malondialdehyde-adduct immunological response as a possible marker of successful aging

Role of PKC-δ activity in glutathione-depleted neuroblastoma cells

Role of PKC-dependent pathways in HNE-induced cell protein transport and secretion

Signal pathway responsible for hepatocyte preconditioning by nitric oxide

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