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Quantitative PCR (qPCR) of human T‐cell leukemia virus type 1 (HTLV‐1) provirus is used for HTLV‐1 testing and for assessment of risk of HTLV‐1‐related diseases. In this study, a reference material was developed for standardizing HTLV‐1 qPCR. Freeze‐dried TL‐Om1 cells diluted with Jurkat cells were prepared and an assigned value for proviral load (PVL) of 2.71 copies/100 cells was determined by digital...
The synthesis of a novel unimolecular reverse micelle, the hyperbranched d-glucan carbamate (3), was accomplished through the carbamation reaction of the hyperbranched d-glucan (1) with the N-carbonyl l-leucine ethyl ester (2) in pyridine at 100°C. Polymer 3 was soluble in a large variety of organic solvents, such as methanol, acetone, chloroform, and ethyl acetate, and insoluble in water, which remarkably...
4-Vinylbenzyl glucoside peracetate (1) was copolymerized with divinylbenzene (DVB) using 1-phenyl-1-(2′,2′,6′,6′-tetramethyl-1′-piperidinyloxy)ethane (2) as an initiator in m-xylene at 138°C for 20h ([DVB]/[2]=28; [DVB]=0.62molL −1 ). The copolymerizations were performed using the mole fraction of 1 in the total feed of 1 and DVB (F 1 : [1]/[1]+[DVB]) ranging from 0.11 to 0.38 that...
SummaryThe reaction of the carboxymethyl cellulose sodium salt (Na-CMC) (degrees of substitution (DS) = 1.2) with N-hydroxysuccinimide (Su-OH) in the presence of 1-hydroxybenzotriazole and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) was carried out in water to obtain the Su-OH ester of carboxymethyl cellulose, Su-CMC, with the DS values of 0.19 – 1.04. N-Allylcarbamoylmethyl cellulose (Allyl-CMC),...
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